Team:UT Dallas/week9

From 2011.igem.org

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<h2>Week 8</h2>
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<h2>Week 9</h2>
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<tr><td valign='top'>8/28/11</td><td valign='top'>Transformation (DH5-alpha): GFP-RBS-TetR-term<br>
<tr><td valign='top'>8/28/11</td><td valign='top'>Transformation (DH5-alpha): GFP-RBS-TetR-term<br>

Latest revision as of 00:54, 28 September 2011

Week 9

8/28/11Transformation (DH5-alpha): GFP-RBS-TetR-term
Incubation of CheZ, CheZ*
  
8/29/11 Glycerol/Miniprep ctx-CheZ, ctx-CheZ*

Digestion: ToxR, FGFR

Incubation ToxR
  
8/30/11 Test Digestion: ctx-CheZ, ctx-Chez*, CheZ, CheZ*

Gel Electrophoresis: ctx-CheZ, ctx-Chez*

Incubation: ToxR
  
8/31/11 Miniprep: ToxR

Full Digestion: ToxR, pSB1C3
Gel Electrophoresis/Purifcation: ToxR,GFP -> CheZ*, FGFR, RBS-TetR-Term

Transformation: Ctx-CheZ*, ToxR-FGFR, GFP-RBS-TetR-Term
  
9/1/11 Plating: k422007 CheY

Incubation: GFP-RBS-TetR-Term (Carb)
Incubation: E.Coli FHK12 - from Germany (Carb)

Incubation: CheZ* (Chlora)
  
9/2/11 Plates Made: CarbR, ChloraR, CarbR/ChloraR

Broth Made: LB CarbR, LB Carb/ChloraR
Miniprep: GFP-RBS-TetR-Term, CheZ*
Glycerol Stock: GFP-RBS-TetR-Term
Made Competent Cells: FHK12 (Carb)
Test Digestion/Gel Electrophoresis: GFP-RBS-TetR-Term,
Full Digestion: CheZ*, FGFR
Ful Digestion: GFP-RBS-TetR-Term
Transformation: German Parts=[pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR
Plasmid stock: Diluted German parts (as above)
Ligation 10 Min: ctx, ToxR into CheZ*, FGFR respectively
Transformation: CheZ*, ctx-CheZ*, FGFR, ToxR-FGFR
Ligation Overnight: Vectors= GFP-RBS-TetR-Term, PyeaR, scp

Inserts= ctx, GFP-RBS, TetR-Term
  
9/3/11 Transformation of the followings:
ctx-GFP-RBS-TetR-Term, PyeaR-GFP-RBS-TetR-Term, scp-GFP-RBS-TetR-Term