Team:Nevada/Project
From 2011.igem.org
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+ | Figure 1. Confirmation of invertase operon parts. PCR was performed to amplify petBD+RBS, KanR, and inv with 20 bp flanking overlap regions. Equimolar volumes of each PCR product were mixed and ran on 0.7% agarose gel at 110 V for 60 minutes (lanes 2-9). 0.5 ug Kb+ standard was loaded into lanes 1 and 10. | ||
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+ | Figure 2. Confirmation of agp in TopoIIBluntPCR vector. Agp was ligated into TopoIIBluntPCR vector (Invitrogen). 0.25 ug of each ligation was digested with SpI and PstI at a final concentration and ran on 0.7% agarose gel at 110 V for 60 minutes (lanes 2-8). 0.5 ug Kb+ standard was loaded into lanes 1 and 9. Lanes 3,5, and 8 indicated successful ligation. | ||
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{{Nevada_Sponsers_CSS}} | {{Nevada_Sponsers_CSS}} |
Revision as of 23:56, 27 September 2011
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