Team:Calgary/Notebook/Protocols/Process20

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Revision as of 22:48, 27 September 2011


Preparing Glycerol Stocks

Reagents and Materials

  • Overnight bacterial growth
  • screw captubes
  • glycerol

Protocol

  1. Pipet 0.5mL of 50% glycerol into 3 1.5 screw cap tubes
  2. Add 0.5mL of overnight culture to each tube
  3. Pipet up and down to gently mix
  4. Flash freeze in liquid N2 or dry ice/ethanol bath
  5. Place in -80 C freezer when frozen

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