Team:Minnesota/davis950-09092011121301-119

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<br>== Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8 ==
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== Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8 ==
'''Project:''' Regulatory
'''Project:''' Regulatory
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<nowiki>Results: None</nowiki>
<nowiki>Results: None</nowiki>
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{{Team:Minnesota/Bottom}}
{{Team:Minnesota/Bottom}}

Latest revision as of 22:46, 27 September 2011

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Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8

Project: Regulatory

Date: Fri, 09 Sep 2011 12:13:01 GMT

Author: Nathan Davis (davis950)

Access: Public

Revision History:

  • Fri, 09 Sep 2011 12:19:11 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)
  • Fri, 09 Sep 2011 12:19:30 GMT (davis950): entry data updated by davis950 (id=2)

Purpose: to digest pUC-pcyA-ho1 and pUC-pTet-Cph8 with appropriate restriction enzymes to put pTet-Cph8 in pUC-pcyA-ho1.


Procedure:


The following digestion reactions were prepared:


  • 16 ul ddH2O
  • 1 ul SpeI
  • 1 ul PstI
  • 3 ul NE buffer 2
  • 10 ul pUC-pcyA-ho1 miniprep product (139 ng/ul)


  • 16 ul ddH2O
  • 1 ul XbaI
  • 1 ul PstI
  • 3 ul NE buffer 2
  • 10 ul pUC-pTet-Cph8 (105 ng/ul)


Digest reactions placed in 37 degree C incubator overnight.


Results: None


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