Team:Nevada/Project
From 2011.igem.org
Davidkoehn88 (Talk | contribs) (→Ethanol Production) |
Davidkoehn88 (Talk | contribs) (→Construct Components) |
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'''1. Primer Design:''' Forward and reverse primers for each DNA part were designed with 20 base pair overlapping sequences with the upstream and downstream flanking segments. This will create 40 base pair overlaps between neighboring parts in the construct.<br> | '''1. Primer Design:''' Forward and reverse primers for each DNA part were designed with 20 base pair overlapping sequences with the upstream and downstream flanking segments. This will create 40 base pair overlaps between neighboring parts in the construct.<br> | ||
- | '''2. PCR:''' | + | '''2. PCR:''' Inv, KnR, and petBD+RBS were amplified from BBa_K558006(Nevada), pUC4K, and K390015(Utah State) respectively under standard PCR conditions. |
- | + | ||
- | Inv, KnR, and petBD+RBS were amplified from BBa_K558006(Nevada), pUC4K, and K390015(Utah State) respectively under standard PCR conditions. | + | |
AGP in pSB1A3 will be amplified to include the vector. This will be used as the backbone for Gibson Assembly. | AGP in pSB1A3 will be amplified to include the vector. This will be used as the backbone for Gibson Assembly. |
Revision as of 22:23, 27 September 2011
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