Team:Calgary/Notebook/Protocols/Process3
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<li type="1">Add the following to a 0.2mL thin-walled PCR tube sitting on ice:</li> | <li type="1">Add the following to a 0.2mL thin-walled PCR tube sitting on ice:</li> | ||
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<div style="margin-bottom:60px"> | <div style="margin-bottom:60px"> | ||
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<td><b>Component</b></td> | <td><b>Component</b></td> | ||
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- | <td>RNA (40ng – 10ng to 1μg total RNA | + | <td>RNA (40ng – 10ng to 1μg total RNA)</td> |
<td>variable</td> | <td>variable</td> | ||
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<li type="1">Incubate in a PCR machine using the following conditions:</li> | <li type="1">Incubate in a PCR machine using the following conditions:</li> | ||
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<tr> | <tr> | ||
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</table> | </table> | ||
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<td><CENTER>5 min at 25°C</CENTER></td> | <td><CENTER>5 min at 25°C</CENTER></td> |
Revision as of 21:46, 27 September 2011
cDNA Synthesis/Reverse Transcription Reaction
Component | Volume |
RNA (40ng – 10ng to 1μg total RNA) | variable |
Nuclease free water | variable |
qScript cDNA synthesis mix | 4 uL |
Final Volume | 20 uL |