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Team:Penn State/Research
From 2011.igem.org
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| - | <p> We | + | <h2>Testing of Or as a Promoter</h2> |
| + | <p>The backbone of the radiation sensor in our project relies on the operating region Or, and the two promoters one on each end of Or. In order to test the function ability of the of the pr promoter we attached a part called mcherry, which has rfp to the promoter. We used the TECAN in our lab to measure the fluorescence of our samples over time. The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional.</p> | ||
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| + | <img src="https://static.igem.org/mediawiki/2011/b/b5/PSUOrTest.png"/ width="100%"> | ||
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Revision as of 02:28, 28 September 2011
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Achievements
We did lots of great things! Check it!
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Registered Team, had (too much) fun throughout the summer |
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Attended the Jamboree |
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At least one submitted and characterized part |
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Demonstrated part works as expected |
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Characterized one new part and entered the data in the “Main Page” section of that Part’s/Device’s Registry entry |
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Worked towards improving the function of an existing BioBrick Part |
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Helped another team (see our collaboration with NYC wetware below!) |
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New approach to human practices (Watch our Human Practices Video and learn about our workshop here) |
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Experimental Results
Testing of Or as a Promoter
The backbone of the radiation sensor in our project relies on the operating region Or, and the two promoters one on each end of Or. In order to test the function ability of the of the pr promoter we attached a part called mcherry, which has rfp to the promoter. We used the TECAN in our lab to measure the fluorescence of our samples over time. The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional.
Dosimeter Design
We will put stuff here
