"
Team:UT Dallas/week7
From 2011.igem.org
(Difference between revisions)
(Created page with "<h2>Week 7</h2> <table> <tr><td valign='top'>8/21/11</td><td valign='top'>Incubation: GFP</td></tr> <tr><td valign='top'> </td><td valign='top'> </td></tr> <tr><td vali...") |
|||
| Line 6: | Line 6: | ||
Test Digestion + Gel Electrophoresis: GFP<br> | Test Digestion + Gel Electrophoresis: GFP<br> | ||
Dissolving/Diluting Primers for ctx promoter and FGFR<br> | Dissolving/Diluting Primers for ctx promoter and FGFR<br> | ||
| - | PCR/Gel purification: ctx</td> | + | PCR/Gel purification: ctx</td></tr> |
<tr><td valign='top'> </td><td valign='top'> </td></tr> | <tr><td valign='top'> </td><td valign='top'> </td></tr> | ||
<tr><td valign='top'>8/23/11</td><td valign='top'>Full Digestion: CheZ, CheZ*, ctx promoter<br> | <tr><td valign='top'>8/23/11</td><td valign='top'>Full Digestion: CheZ, CheZ*, ctx promoter<br> | ||
Dissolving/Diluting Primers: -----------------------------<br> | Dissolving/Diluting Primers: -----------------------------<br> | ||
Gel Electrophoresis/ Purification of CheZ, CheZ*<br> | Gel Electrophoresis/ Purification of CheZ, CheZ*<br> | ||
| - | Ligation: ctx with CheZ, CheZ*</td> | + | Ligation: ctx with CheZ, CheZ*</td></tr> |
<tr><td valign='top'> </td><td valign='top'> </td></tr> | <tr><td valign='top'> </td><td valign='top'> </td></tr> | ||
<tr><td valign='top'>8/24/11</td><td valign='top'>PCR Point Mutation: FGFR (get rid of EcoRI restriction site)<br> | <tr><td valign='top'>8/24/11</td><td valign='top'>PCR Point Mutation: FGFR (get rid of EcoRI restriction site)<br> | ||
Transformation (XL10 ultra competent): FGFR (point mutated) <br> | Transformation (XL10 ultra competent): FGFR (point mutated) <br> | ||
| - | Transformation: Ctx-CheZ (Carb), ctx-CheZ* (Chlora)</td> | + | Transformation: Ctx-CheZ (Carb), ctx-CheZ* (Chlora)</td></tr> |
<tr><td valign='top'> </td><td valign='top'> </td></tr> | <tr><td valign='top'> </td><td valign='top'> </td></tr> | ||
<tr><td valign='top'>8/25/11</td><td valign='top'>Incubation: FGFR | <tr><td valign='top'>8/25/11</td><td valign='top'>Incubation: FGFR | ||
Digestion, Gel Electrophoresis/Purification: CheZ, CheZ*<br> | Digestion, Gel Electrophoresis/Purification: CheZ, CheZ*<br> | ||
| - | Ligation Overnight: Ctx --> CheZ, CheZ*</td> | + | Ligation Overnight: Ctx --> CheZ, CheZ*</td></tr> |
<tr><td valign='top'> </td><td valign='top'> </td></tr> | <tr><td valign='top'> </td><td valign='top'> </td></tr> | ||
<tr><td valign='top'>8/26/11</td><td valign='top'>Glycerol Stock/Miniprep: FGFR<br> | <tr><td valign='top'>8/26/11</td><td valign='top'>Glycerol Stock/Miniprep: FGFR<br> | ||
Transformation CheZ, CheZ*<br> | Transformation CheZ, CheZ*<br> | ||
Test Digestion, Gel Electrophoresis<br> | Test Digestion, Gel Electrophoresis<br> | ||
| - | Made LbB Chlora & LB Kan</td> | + | Made LbB Chlora & LB Kan</td></tr> |
<tr><td valign='top'> </td><td valign='top'> </td></tr> | <tr><td valign='top'> </td><td valign='top'> </td></tr> | ||
<tr><td valign='top'>8/27/11</td><td valign='top'>Digestion, Gel Electrophoresis/Purification: RBS-TetR, GFP<br> | <tr><td valign='top'>8/27/11</td><td valign='top'>Digestion, Gel Electrophoresis/Purification: RBS-TetR, GFP<br> | ||
Ligation (10 min): GFP into RBS-TetR-double term<br> | Ligation (10 min): GFP into RBS-TetR-double term<br> | ||
| - | Transformation: GFP-RBS-TetR-term</td> | + | Transformation: GFP-RBS-TetR-term</td></tr> |
</table> | </table> | ||
Revision as of 22:06, 27 September 2011
Week 7
| 8/21/11 | Incubation: GFP |
| 8/22/11 | Glycerol Stock/Miniprep GFP Test Digestion + Gel Electrophoresis: GFP |
| 8/23/11 | Full Digestion: CheZ, CheZ*, ctx promoter Dissolving/Diluting Primers: ----------------------------- |
| 8/24/11 | PCR Point Mutation: FGFR (get rid of EcoRI restriction site) Transformation (XL10 ultra competent): FGFR (point mutated) |
| 8/25/11 | Incubation: FGFR
Digestion, Gel Electrophoresis/Purification: CheZ, CheZ* |
| 8/26/11 | Glycerol Stock/Miniprep: FGFR Transformation CheZ, CheZ* |
| 8/27/11 | Digestion, Gel Electrophoresis/Purification: RBS-TetR, GFP Ligation (10 min): GFP into RBS-TetR-double term |
