"
Team:Calgary/Notebook/Protocols
From 2011.igem.org
(Difference between revisions)
Emily Hicks (Talk | contribs) |
Emily Hicks (Talk | contribs) |
||
| Line 23: | Line 23: | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process13">Bacterial Transformation</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process13">Bacterial Transformation</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process14">Rehydration of Registry DNA</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process14">Rehydration of Registry DNA</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process15">Restriction Digest</a></li> | ||
</ul> | </ul> | ||
</html> | </html> | ||
}} | }} | ||
Revision as of 21:36, 27 September 2011
Notebook
Post-Regionals
Journal
- Defining Objectives
- Techniques Workshop
- Bioinformatics & qRT-PCR
- Sensory Element Fishing
- Electrochem - CPR Oxidation
- Electrochem - Reporter Gene
- Pseudomonas Tools
- Microalgae Tools
- NA Viability Assays
- May - July Group Journal
Protocols
Safety
Protocols
Hello! Our wiki is currently under heavy construction, so please check regularly for updates. Thank you for your patience.
Here is a list of the procedures we used this summer. Each one has a description and list of materials needed.
- Plasmid Extraction
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Effectiveness of the pBAD Construct
- Preparation of Algal f2 Media
- Glass Bead Algal transformation
- Chloroplast Isolation
- Nuclear DNA extraction from Algae
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Inudcing E. coli to produce β-galactosidase
- Bacterial Transformation
- Rehydration of Registry DNA
- Restriction Digest
