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| | As part of this project, we are developing a yeast expression platform including a library of promoters, termination sequences, and shuttle vectors. While iGEM has many well-characterized basic parts for ''E. Coli'', yeast remains a largely untapped resource. The inclusion of shuttle vectors in our platform will allow future genetic engineers to take advantage of ''E. coli'''s ability to rapidly replicate while still being able to deploy their construct in ''S. cerevisiae''. We hope our work will facilitate the use of yeast in future iGEM projects. | | As part of this project, we are developing a yeast expression platform including a library of promoters, termination sequences, and shuttle vectors. While iGEM has many well-characterized basic parts for ''E. Coli'', yeast remains a largely untapped resource. The inclusion of shuttle vectors in our platform will allow future genetic engineers to take advantage of ''E. coli'''s ability to rapidly replicate while still being able to deploy their construct in ''S. cerevisiae''. We hope our work will facilitate the use of yeast in future iGEM projects. |
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| - | We aimed to add the Violacein pathway to yeast using yeast promoters and UTR's. It will be the first time the violacein pathway will be expressed in a eukaryotic organism. For further details on the violacein purple pigment, please visit the [https://2009.igem.org/Team:Cambridge/Project/VI01 "Violacein Page"] on the Cambridge iGEM 2009 website. | + | We aim to add the Violacein pathway to yeast using yeast promoters and 3'UTR's. It will be the first time the violacein pathway will be expressed in a eukaryotic organism. For further details on the purple pigment violacein, please visit the [https://2009.igem.org/Team:Cambridge/Project/VI01 "Violacein Page"] on the Cambridge iGEM 2009 website. |
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Yeast Toolkit Background
As part of this project, we are developing a yeast expression platform including a library of promoters, termination sequences, and shuttle vectors. While iGEM has many well-characterized basic parts for E. Coli, yeast remains a largely untapped resource. The inclusion of shuttle vectors in our platform will allow future genetic engineers to take advantage of E. coli's ability to rapidly replicate while still being able to deploy their construct in S. cerevisiae. We hope our work will facilitate the use of yeast in future iGEM projects.
We aim to add the Violacein pathway to yeast using yeast promoters and 3'UTR's. It will be the first time the violacein pathway will be expressed in a eukaryotic organism. For further details on the purple pigment violacein, please visit the "Violacein Page" on the Cambridge iGEM 2009 website.
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