From 2011.igem.org
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- | We are focused on creating a self sustaining biofuel production system. To accomplish this we must co-cultivate two different types of bacteria that will eventually lead to the production of ethanol and fatty acids used for biofuel. The idea is based upon using a photosynthetic bacteria to feed E. coli which will then produce the ethanol and fatty acids. This would drastically reduce the cost of production of biofuels, which is the main problem we have with them as a viable energy source at this time. This also has implications for the drug industry as many medicines, such as insulin, are manufactured from E.coli bacteria. <br>To get this system to work we must genetically engineer the different bacteria to allow us to change their natural production processes. With the photosynthetic cyanobacteria we will be incorporating 2 new genes as well as knocking out 2 that already exist in the bacteria. This will allow us to produce the maximum amount of glucose and secrete it outside the cell. The E.coli will then feed off this glucose and use it to grow and produce ethanol.<br>In engineering E.coli we will be incorporating 3 genes. PDC/ADH and BTE. These are genes that regulate and control metabolic pathways and will lead to the production of ethanol and fatty acids respectively. Our two bacteria will then be grown together in a new system that we have designed to allow for the exchange of glucose through a semi-permeable membrane. E.coli will feed off this glucose while producing ethanol and biodiesel which we can extract from our production chamber.
| + | A Cooperative Relationship between Cyanobacteria and E.Coli |
| + | for production of Biofuels |
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| | | |
Revision as of 22:27, 26 September 2011
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/* Thanks guys - Bill Collins */
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