Team:UEA-JIC Norwich/Notebook

From 2011.igem.org

(Difference between revisions)
Line 1: Line 1:
<div style="background-color:#6AFB92; color:#254117">
<div style="background-color:#6AFB92; color:#254117">
<html>
<html>
-
<head>
+
 
<style type="text/css">
<style type="text/css">
-
ul
+
#globalWrapper {background-color: #153E7E;}
-
{
+
#top-section { height: 14px; margin: 0px; margin-left: auto; margin-right: auto; margin-bottom: 0 !important;}
-
list-style-type:none;
+
#p-logo { height: 0px; margin: -1px; margin-left: auto; margin-right: auto; margin-bottom: 0 !important;}
-
margin:0;
+
#menubar.left-menu { background-color:#606060; width:965px; float:left; border: none;}
-
padding:0;
+
#menubar.left-menu a {color:white;}
-
padding-top:6px;
+
#search-controls {display:none; }
-
padding-bottom:6px;
+
#contentgrid {padding-left:25px;padding-right:25px;width:925px;border: 1px solid #529bc7background-color: white; margin-left:-6px; margin-bottom:-5px; }
-
}
+
.firstHeading{width: 0px; height: 0px; margin-bottom: 0px; display: none; position: relative; top:0; left:0; margin:0;}  
-
li
+
-
{
+
-
display:inline;
+
-
}
+
-
a:link,a:visited
+
-
{
+
-
font-weight:bold;
+
-
color:#254117;
+
-
text-align:center;
+
-
padding:6px;
+
-
text-decoration:none;
+
-
text-transform:uppercase;
+
-
}
+
-
a:hover,a:active
+
-
{
+
-
background-color:#387C44;
+
-
}
+
 +
#headerimage {width:965px; margin-bottom:10px}
 +
#headerimage img {max-width:965px;}
 +
#headermenu {text-align:center; width:965px; height:22px;border-bottom:1px solid #529bc7;}
 +
#headermenu ul {display:inline-block; zoom:1;}
 +
#headermenu ul.top {margin-left:0;}
 +
#headermenu ul {text-align:left; margin:0; padding:0; list-style:none; white-space:nowrap;}
 +
#headermenu li {margin:0; padding:0;}
 +
#headermenu a {display:block; font:normal 11px verdana,arial,sans-serif;color:#82CAFA; line-height:22px; text-decoration:none; padding:0 20px;}
 +
#headermenu li:hover > ul {visibility:visible;}
 +
#headermenu a:hover ul,
 +
#headermenu a:hover a:hover ul,
 +
#headermenu a:hover a:hover a:hover ul {visibility:visible;}
 +
#headermenu a:hover ul ul,
 +
#headermenu a:hover a:hover ul ul {visibility:hidden;}
 +
#headermenu ul.top {margin:0 auto;}
 +
#headermenu li.top-li {float:left; position:relative; margin-right:1px;}
 +
#headermenu a.top-a {float:left; padding:0 0 0 20px; background:url(https://static.igem.org/mediawiki/2011/2/2f/Screen_shot_2011-06-23_at_14.05.42.png) no-repeat left top;}
 +
#headermenu a.top-a b {float:left; padding:0 20px 0 0; background:url(https://static.igem.org/mediawiki/2011/2/22/Screen_shot_2011-06-23_at_14.01.36.png) no-repeat right top; cursor:pointer; cursor:hand;}
 +
#headermenu a.down b {float:left; padding:0 20px 0 0; background:url(https://static.igem.org/mediawiki/2011/2/22/Screen_shot_2011-06-23_at_14.01.36.png) no-repeat right top; cursor:pointer;}
 +
#headermenu a.top-a:hover {white-space:nowrap; background:url(https://static.igem.org/mediawiki/2011/2/2f/Screen_shot_2011-06-23_at_14.05.42.png) no-repeat left -30px;}
 +
#headermenu a.top-a:hover b,
 +
#headermenu a.top-a:focus b,
 +
#headermenu a.top-a:active b {color:#FDD017; background:url(https://static.igem.org/mediawiki/2011/3/33/Tab_new_1.png) no-repeat right -30px; cursor:pointer;}
 +
#headermenu a.down:hover b,
 +
#headermenu a.down:focus b,
 +
#headermenu a.down:active b {color:#000; background:url(https://static.igem.org/mediawiki/2011/3/33/Tab_new_1.png) no-repeat right -30px; cursor:pointer;}
 +
#headermenu li.top-li:hover > a {white-space:nowrap; background:url(https://static.igem.org/mediawiki/2011/d/d7/0.5_Tab.png) no-repeat left -30px;}
 +
#headermenu li.top-li:hover > a b {color:#FDD017;  background:url(https://static.igem.org/mediawiki/2011/3/33/Tab_new_1.png) no-repeat right -30px;}
 +
#headermenu li.top-li:hover > a.down b {color:#FDD017;  background:url(https://static.igem.org/mediawiki/2011/3/33/Tab_new_1.png) no-repeat right -30px;}
 +
#headermenu li ul {display:block; position:absolute; visibility:hidden; background:#529bc7; padding:1px 1px 8px 1px; left:0;}
 +
#headermenu li li {border-bottom:1px solid #529bc7;}
 +
#headermenu li li a {background:#fff;}
 +
#headermenu li li a:hover {background:#153E7E;}
 +
#headermenu li li:hover > a {background:#153e7e;}
 +
#headermenu ul.drop-down {top:22px; opacity:1.0;}
 +
#headermenu li li ul {left:100%; margin-top:-23px; margin-left:-5px;}
 +
#headermenu table {text-align:left; position:absolute;top:0;left:0;border-collapse:collapse;}
 +
#headermenu table ul li a {padding-left:0; padd\ing-left:20px;}
 +
#headermenu table table {top:auto; left:100%; margin-left:-1px; padding:0; margin:0;}
 +
#headermenu table table ul {margin-top:-4px; marg\in-top:-7px;}
</style>
</style>
-
</head>
+
<div id="wrap">
 +
<div id="headermenu">
 +
<ul class="top">
 +
<li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich"><b>Home</b></a>
 +
</li>
 +
       
 +
<li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Team"><b>Team</b></a>
 +
 +
</li>
 +
       
 +
<li class="top-li"><a class="top-a down" href=""><b>Aims</b>
 +
<ul class="drop-down">
 +
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Project">Overview</a></li>
 +
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Nittygritty">The 'Nitty Gritty'</a></li>
 +
 +
</ul>
 +
</li>
 +
       
 +
<li class="top-li"><a class="top-a down" href=""><b>Registry Parts</b>
 +
<ul class="drop-down">
 +
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Registryoverview">Overview</a></li>
 +
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Registrycharacterisation">Characterization</a></li>
 +
</ul>
 +
</li>
 +
       
 +
<li class="top-li"><a class="top-a down" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Methods"><b>Methods</b>
 +
 +
</li>
-
<body>
+
<li class="top-li"><a class="top-a down" href=""><b>Journal</b>
-
<ul>
+
<ul class="drop-down">
-
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich">Home</a></li>
+
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Notebook">Lab Journal</a></li>
-
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Team">The Team</a></li>
+
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/DaytoDay">Day to Day Journal</a></li>
-
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Project">Aims</a></li>
+
</ul>
-
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Parts">Parts Submitted To The Registry</a></li>
+
</li>
-
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Methods">Methods</a></li>
+
     
-
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Notebook">Lab Journal</a></li>
+
-
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Safety">Lab Safety</a></li>
+
-
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Follow us">Follow Us</a></li>
+
-
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Outreach">Outreach</a></li>
+
-
</ul>
+
-
</body>
+
 +
<li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Safety"><b>Lab Safety</b></a>
 +
</li> 
 +
<li class="top-li"><a class="top-a" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Follow_us"><b>Follow Us</b></a>
 +
</li> 
 +
<li class="top-li"><a class="top-a down" href=""><b>Human Practices</b></a>
 +
<ul class="drop-down">
 +
<li><a href="top-a down" href="https://2011.igem.org/Team:UEA-JIC_Norwich/Outreach">Outreach</a></li>
 +
<li><a href="https://2011.igem.org/Team:UEA-JIC_Norwich/Energyconservation">Energy Conservation</a></li>
 +
</ul></li> 
 +
  </div>
 +
<html>
 +
 +
<div id="contentgrid">
</html>
</html>
 +
<html>
<html>
<head>
<head>

Revision as of 14:39, 23 June 2011

Lab Journal

Monday 20th June - Attempted transformations of E.coli with biobricks for the first time. We used the Banana odour generator; red and green luciferase; and Epic biobricks. We followed the protocol offered by the New England Biolabs for this procedure (see methods). We find out tomorrow if it worked!

Tuesday 21st June- The control cells and 'Epic' Biobrick cells were successfully transformed. The cells transformed with the 'Banana odour' Biobrick mysteriously vanished from the incubator and were found a day later in the chill room. All other transformations failed. We repeated protocols of all unsuccessful transformations.

Wednesday 22nd June- All transformations successful. Much relief. Made cultures from colonies of all transformations with the aim of making glycerol stock solutions as soon as possible.

Standard Journal

Wednesday 22nd June - Told last night after going to see X-men that we need to have a poster completed and ready for use by friday. Spent the day writing poster drafts and designing the layout, as well as attempting to copy the logo we designed onto the computer.

Retrieved from "http://2011.igem.org/Team:UEA-JIC_Norwich/Notebook"