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Team:Panama/Notebook/June2011
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3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium. | 3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium. | ||
| - | 4. Last attempt | + | 4. Last attempt to inoculate the E. coli containing the rh1AB mutated from 2/3/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol (33 ug/ml). |
5. We did all the necessary calculations for the antibiotics concentrations. | 5. We did all the necessary calculations for the antibiotics concentrations. | ||
Latest revision as of 03:58, 27 September 2011
Notebook - June 2011
June 29, 2011
We did a lot of work today like:
1. The miniprep of the strains containing the BioBrick rh1AB.
2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA.
3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium.
4. Last attempt to inoculate the E. coli containing the rh1AB mutated from 2/3/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol (33 ug/ml).
5. We did all the necessary calculations for the antibiotics concentrations.
Rh1AB + psB1C3 Ectroforesis
1 and 8: Marker 100- 3000 pb. 2-7: rh1AB 2010 BioBrick
