Team:ZJU-China/Notebook
From 2011.igem.org
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Revision as of 15:15, 26 September 2011
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Week1
Day | Note | ||
Jul.4th Monday |
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Jul 5th Tuesday | |||
Jul.6th Wednesday |
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Jul.7th Thursday |
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Jul.8th Friday |
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Jul.9th Saturday |
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Jul.10th Sunday |
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Data&protocol
Monday
There is no data in first week
Week2
Day | Note | |||||
Jul.11th Monday |
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Jul 12th Tuesday |
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Jul.13th Wednesday |
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Jul.14th Thursday |
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Jul.15th Friday |
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Jul.16th Saturday |
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Jul.17th Sunday |
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Data&protocol
Jul.13th Wednesday
Systems of restriction digestion with EcoRI and PstI
Plasmid | 1μL (>100ng) |
EcoRI | 1μL |
PstI | 1μL |
10×Buffer Tango | 2μL |
ddH2O | 15μL |
Temperature grad
56℃,57.4℃,60.2℃,62.9℃,64.3℃,65.8℃
PCR system (test the Tm of the primers CP1&CS, NP&NS)
10×Buffer | 2μL |
dNTPs | 0.5μL |
primers CP1 (NP) | 1μL |
primers CS (NS) | 1μL |
Template | 1μL |
rTaq | 0.2μL |
H2O | 14.5μL |
Total | 20μL |
Jul.14th Thursday
PCR systems(test the Tm of the primers for YFP,RFP,tetR,Vgb and nirB )
10×Buffer | 2μL |
dNTPs | 0.5μL |
primers F | 0.5μL |
primers R | 0.5μL |
Template | 1μL |
Taq | 0.2μL |
H2O | 15.5μL |
Total | 20μL |
Temperature grad and the results:
vgb&nirB pcr test:
Week3
Day | Note | ||||
Jul.18th Monday |
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Jul 19th Tuesday |
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Jul.20th Wednesday |
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Jul.21th Thursday |
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Jul.22th Friday |
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Jul.23th Saturday |
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Jul.24th Sunday |
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Data&protocol
Monday
WEEK3
Tuesday
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Week4
Day | Note | ||||
Jul.25th Monday |
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Jul. 26th Tuesday |
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Jul.27th Wednesday |
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Jul.28th Thursday |
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Jul.29th Friday |
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Jul.30th Saturday | |||||
Jul.31th Sunday |
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Data&protocol
Monday
WEEK3
Tuesday
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Week5
Day | Note | ||||
Aug.1st Monday |
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Aug.2nd Tuesday |
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Aug.3rd Wednesday |
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Aug.4th Thursday |
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Aug.5th Friday |
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Aug.6th Saturday | |||||
Aug.7th Sunday |
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Data&protocol
Monday
WEEK3
Tuesday
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Week6
Day | Note | ||||
Aut.8th Monday |
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Aug.9th Tuesday |
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Aug.10th Wednesday |
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Aug.11th Thursday |
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Aug.12th Friday |
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Aug.13th Saturday |
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Aug.14th Sunday |
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Data&protocol
Monday
WEEK3
Tuesday
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Data&protocol
Monday
WEEK3
Tuesday
bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbb
Data&protocol
Monday
WEEK3
Tuesday
bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbb
Data&protocol
Monday
WEEK3
Tuesday
bbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbbb
Data&protocol
Monday
WEEK3
Tuesday
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