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Team:Greenfield IN-Rihm-HS/May 25, 2011
From 2011.igem.org
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1. Isolation of DNA from yeast with primers for Gibson and primers for iGEM submission | 1. Isolation of DNA from yeast with primers for Gibson and primers for iGEM submission | ||
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2. iGEM insertion for submission, using Gibson | 2. iGEM insertion for submission, using Gibson | ||
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3. Gibson assembly into plasmid 416ura3 | 3. Gibson assembly into plasmid 416ura3 | ||
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4. Transform into e.coli | 4. Transform into e.coli | ||
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5. Transform into yeast | 5. Transform into yeast | ||
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6. Isolation of protein, Work on field process, Work on concentration controls | 6. Isolation of protein, Work on field process, Work on concentration controls | ||
Revision as of 04:33, 23 June 2011
Checked on primers
Did a laboratory check for procedures,materials, and safety protocols
Mapped out the next three weeks in the lab
1. Isolation of DNA from yeast with primers for Gibson and primers for iGEM submission
2. iGEM insertion for submission, using Gibson
3. Gibson assembly into plasmid 416ura3
4. Transform into e.coli
5. Transform into yeast
6. Isolation of protein, Work on field process, Work on concentration controls
