"
Team:Kyoto/Hunger
From 2011.igem.org
(→Project Hunger) |
|||
| Line 12: | Line 12: | ||
<br>http://partsregistry.org/wiki/index.php/Part:BBa_J64978 | <br>http://partsregistry.org/wiki/index.php/Part:BBa_J64978 | ||
<br>などがもう登録されているようですが | <br>などがもう登録されているようですが | ||
| + | <br>リンク先を見ましたがどうなんでしょうね とりあえずこの登録されているパーツのシーケンスは見ましたが今回我々が作ろうとしたglnLとは全然違うシーケンスでした 名に由来かは知りませんがE.coliのものじゃないでしょうねby Hashiya | ||
== '''Introduction''' == | == '''Introduction''' == | ||
For every living thing, needless biological activity is not efficient. Cells must be controlled so that enzymes are produced only when they are necessary. Ammonia is an essential nitrogen source for the bactria. When enteric bacteria are deprived of ammonia, they express glnA to produce glutamine synthetase(GS). Nitrogen is used in the reaction of | For every living thing, needless biological activity is not efficient. Cells must be controlled so that enzymes are produced only when they are necessary. Ammonia is an essential nitrogen source for the bactria. When enteric bacteria are deprived of ammonia, they express glnA to produce glutamine synthetase(GS). Nitrogen is used in the reaction of | ||
Revision as of 06:05, 26 September 2011
Contents |
Project Hunger
Production of needless enzymes is a heavy burden especially when the resource is scarce. This can be reduced by using nitrogen regulatory proteins, NtrB and NtrC† which activate σ54promoter when the supply of nitrogen is not enough. NtrB and NtrC are coded in glnL and glnG, respectively. Here, we evaluated the relationships between expression under σ54promoter and that of these genes by the aid of RPU(a relative promoter unit).
† NtrB and NtrC are otherwise called NRII, NRI
ところでこの遺伝子はもともとBioBrickに登録されていたものか、今回新たに作ったもののどちらなのでしょう
http://partsregistry.org/wiki/index.php/Part:BBa_J64978
などがもう登録されているようですが
リンク先を見ましたがどうなんでしょうね とりあえずこの登録されているパーツのシーケンスは見ましたが今回我々が作ろうとしたglnLとは全然違うシーケンスでした 名に由来かは知りませんがE.coliのものじゃないでしょうねby Hashiya
Introduction
For every living thing, needless biological activity is not efficient. Cells must be controlled so that enzymes are produced only when they are necessary. Ammonia is an essential nitrogen source for the bactria. When enteric bacteria are deprived of ammonia, they express glnA to produce glutamine synthetase(GS). Nitrogen is used in the reaction of
Glutamate + NH3 + ADP → Glutamine + ADP + phosphate
GS
The expression of glnA is regulated by several proteins including NtrB, NtrC, Pii.
Fig. NtrCがσ54,RNAポリメラーゼ,DNAの関係図(窒素源あり/なし) Fig. NtrBCの関係図 Fig. Ntr, Pii, GSの関係図
