Team:UC Davis/Data LambdacI

From 2011.igem.org

(Difference between revisions)
Line 19: Line 19:
<div class="floatbox">
<div class="floatbox">
-
<h1 id="widget"> λ cI Promoter Characterization Data</h1>
+
<h1 id="widget"> ? cI Promoter Characterization Data</h1>
<div id="mutantwidget" class="floatbox2">
<div id="mutantwidget" class="floatbox2">
-
<h3>Wildtype</h3>
+
 
-
<span class="sequence">SEQUENCE GOES HERE!</span>
+
<h3>Wildtype</h3>
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
<span class="sequence">SEQUENCE GOES HERE!</span>
-
<span class="ydata">
+
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
-
  1.010689,  
+
<span class="ydata"> 0.99566, 0.95671, 0.880328, 0.734557, 0.662629, 0.607491, 0.530352</span>
-
  0.967933,
+
<span class="stdevs"> 0.012905, 0.015917, 0.020404, 0.026943, 0.013849, 0.0068952, 0.0060456</span>
-
  0.880328,  
+
<span class="numconditions">4</span>
-
  0.734557,
+
-
  0.662629,  
+
<h3>Mutant 1</h3>
-
  0.607491,  
+
<span class="sequence">SEQUENCE GOES HERE!</span>
-
  0.530352, 
+
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
-
</span>
+
<span class="ydata"> 0.6492, 0.20526, 0.17329, 0.16899, 0.16682, 0.16631, 0.16913</span>
-
<span class="numconditions">4</span>
+
<span class="stdevs"> 0.017682, 0.0032754, 0.0010138, 0.0011266, 0.00097033, 0.0012072, 0.0011179</span>
-
<!--<span class='xdata_3d'>0, 1, 2, 5</span>
+
 
-
<span class='ydata_3d'>0.00000,   0.20000,   0.40000,   0.60000,   0.80000,   1.00000,  2.00000</span>
+
<h3>Mutant 2</h3>
-
<span class='zdata_3d'>
+
<span class="sequence">SEQUENCE GOES HERE!</span>
-
  0.99315,  1.02097,  0.99023,  0.99354,
+
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
-
  0.78019,  0.97021,  0.96725,  0.96226,
+
<span class="ydata"> 0.53303, 0.16064, 0.14171, 0.12149, 0.12547, 0.11146, 0.1061</span>
-
  0.58405,  0.87100,  0.89795,  0.94529,
+
<span class="stdevs"> 0.02651, 0.0029121, 0.0027198, 0.0033112, 0.0034421, 0.0059837, 0.0027095</span>
-
  0.45400,  0.76333,  0.85071,  0.93618,
+
 
-
  0.35401,  0.65243,  0.75444,  0.88056,
+
<h3>Mutant 3</h3>
-
  0.29099,  0.55454,  0.68266,  0.84441,
+
<span class="sequence">SEQUENCE GOES HERE!</span>
-
  0.13305,  0.26829,  0.37015,  0.55294</span>--!>
+
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
-
<h3>Mutant 1</h3>
+
<span class="ydata"> 0.99566, 0.95671, 0.880328, 0.734557, 0.662629, 0.607491, 0.530352</span>
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
+
<span class="stdevs"> 0.012905, 0.015917, 0.020404, 0.026943, 0.013849, 0.0068952, 0.0060456</span>
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
 
-
<span class="ydata">
+
<h3>Mutant 4</h3>
-
  0.651227,  
+
<span class="sequence">SEQUENCE GOES HERE!</span>
-
  0.207767,
+
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
-
  0.171959,  
+
<span class="ydata"> 0.23, 0.038825, 0.035663, 0.036119, 0.035423, 0.034289, 0.032858</span>
-
  0.165190,
+
<span class="stdevs"> 0.02048, 0.00047265, 0.00028731, 0.00037973, 0.00031652, 0.00026622, 0.00070058</span>
-
  0.160246,  
+
 
-
  0.157574,  
+
<h3>Mutant 5</h3>
-
  0.155748,  
+
<span class="sequence">SEQUENCE GOES HERE!</span>
-
</span>
+
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
-
<h3>Mutant 2</h3>
+
<span class="ydata"> 0.92311, 0.23346, 0.1833, 0.17092, 0.16534, 0.16473, 0.15538</span>
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
+
<span class="stdevs"> 0.0061434, 0.0029621, 0.0016015, 0.0037633, 0.0029015, 0.002096, 0.0024785</span>
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
 
-
<span class="ydata">
+
<h3>Mutant 6</h3>
-
  0.515178,  
+
<span class="sequence">SEQUENCE GOES HERE!</span>
-
  0.148993,
+
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
-
  0.116731,  
+
<span class="ydata"> 0.97182, 0.98592, 1.01000, 1.02300, 1.03610, 1.04490, 1.01750</span>
-
  0.092202,
+
<span class="stdevs"> 0.018621, 0.01966, 0.018634, 0.018826, 0.018993, 0.020762, 0.019842</span>
-
  0.086236,  
+
 
-
  0.085269,  
+
<h3>Mutant 7</h3>
-
  0.081236,  
+
<span class="sequence">SEQUENCE GOES HERE!</span>
-
</span>
+
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
-
<h3>Mutant 3</h3>
+
<span class="ydata"> 0.48325, 0.13919, 0.12353, 0.10617, 0.10943, 0.099213, 0.08565</span>
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
+
<span class="stdevs"> 0.012073, 0.0025601, 0.0030506, 0.0027667, 0.002179, 0.0022998, 0.0015159</span>
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
 
-
<span class="ydata">
+
<h3>Mutant 8</h3>
-
  0.919031,  
+
<span class="sequence">SEQUENCE GOES HERE!</span>
-
  0.234935,
+
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
-
  0.180555,  
+
<span class="ydata"> 0.41657, 0.12325, 0.10744, 0.10365, 0.10006, 0.096006, 0.09219</span>
-
  0.162829,
+
<span class="stdevs"> 0.0080566, 0.0028055, 0.0027614, 0.0038199, 0.0038188, 0.0024541, 0.0022523</span>
-
  0.157128,  
+
-
  0.152808,  
+
-
  0.141317,  
+
-
</span>
+
-
<h3>Mutant 4</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.220505,  
+
-
  0.041865,
+
-
  0.037100,  
+
-
  0.036120,
+
-
  0.035185,  
+
-
  0.034249,  
+
-
  0.032601,  
+
-
</span>
+
-
<h3>Mutant 5</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.919031,  
+
-
  0.234935,
+
-
  0.180555,  
+
-
  0.162829,
+
-
  0.157128,  
+
-
  0.152808,  
+
-
  0.141317,  
+
-
</span>
+
-
<h3>Mutant 6</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.986299,  
+
-
  1.042648,
+
-
  1.069110,  
+
-
  1.085407,
+
-
  1.116565,  
+
-
  1.116565,  
+
-
  1.070768, 
+
-
</span>
+
-
<h3>Mutant 7</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.470486,  
+
-
  0.131343,
+
-
  0.104605,  
+
-
  0.081761,
+
-
  0.075629,  
+
-
  0.067232,  
+
-
  0.062205,  
+
-
</span>
+
-
        <h3>Mutant 8</h3>
+
-
<span class="sequence">caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca</span>
+
-
<span class="xdata">0, .0014, .0028, .0042, .0056, .0070, .0140</span>
+
-
<span class="ydata">
+
-
  0.388973,  
+
-
  0.108555,
+
-
  0.086125,  
+
-
  0.075740,
+
-
  0.065796,  
+
-
  0.059857,  
+
-
  0.053090,  
+
-
</span>
+
</div>
</div>
</div>
</div>
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Revision as of 22:21, 25 September 2011

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Criteria

View our judging criteria for iGEM 2011 here.

? cI Promoter Characterization Data

Wildtype

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.99566, 0.95671, 0.880328, 0.734557, 0.662629, 0.607491, 0.530352 0.012905, 0.015917, 0.020404, 0.026943, 0.013849, 0.0068952, 0.0060456 4

Mutant 1

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.6492, 0.20526, 0.17329, 0.16899, 0.16682, 0.16631, 0.16913 0.017682, 0.0032754, 0.0010138, 0.0011266, 0.00097033, 0.0012072, 0.0011179

Mutant 2

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.53303, 0.16064, 0.14171, 0.12149, 0.12547, 0.11146, 0.1061 0.02651, 0.0029121, 0.0027198, 0.0033112, 0.0034421, 0.0059837, 0.0027095

Mutant 3

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.99566, 0.95671, 0.880328, 0.734557, 0.662629, 0.607491, 0.530352 0.012905, 0.015917, 0.020404, 0.026943, 0.013849, 0.0068952, 0.0060456

Mutant 4

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.23, 0.038825, 0.035663, 0.036119, 0.035423, 0.034289, 0.032858 0.02048, 0.00047265, 0.00028731, 0.00037973, 0.00031652, 0.00026622, 0.00070058

Mutant 5

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.92311, 0.23346, 0.1833, 0.17092, 0.16534, 0.16473, 0.15538 0.0061434, 0.0029621, 0.0016015, 0.0037633, 0.0029015, 0.002096, 0.0024785

Mutant 6

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.97182, 0.98592, 1.01000, 1.02300, 1.03610, 1.04490, 1.01750 0.018621, 0.01966, 0.018634, 0.018826, 0.018993, 0.020762, 0.019842

Mutant 7

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.48325, 0.13919, 0.12353, 0.10617, 0.10943, 0.099213, 0.08565 0.012073, 0.0025601, 0.0030506, 0.0027667, 0.002179, 0.0022998, 0.0015159

Mutant 8

SEQUENCE GOES HERE! 0, .0014, .0028, .0042, .0056, .0070, .0140 0.41657, 0.12325, 0.10744, 0.10365, 0.10006, 0.096006, 0.09219 0.0080566, 0.0028055, 0.0027614, 0.0038199, 0.0038188, 0.0024541, 0.0022523
Some preliminary data. These are LacI promoter mutants. The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter. There's still more to come!

Retrieved from "http://2011.igem.org/Team:UC_Davis/Data_LambdacI"