Team:Tec-Monterrey/projectnotebook june/day08

From 2011.igem.org

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08/June/2011
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• We sterilized all the material for the calcium competent cells
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• Now, we do the calcium competent cells
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• Top 10 and DH5- alpha ->in plates with Km, Ap, without antibiotics
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• Transformation in the microwave of : 16K, 20B, 14N, 22P, 2M
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• We ALMOST burn the lab…. Thanks to Chucho
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</p>

Revision as of 02:16, 28 September 2011

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08/June/2011
• We sterilized all the material for the calcium competent cells
• Now, we do the calcium competent cells
• Top 10 and DH5- alpha ->in plates with Km, Ap, without antibiotics
• Transformation in the microwave of : 16K, 20B, 14N, 22P, 2M
• We ALMOST burn the lab…. Thanks to Chucho