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Team:Tec-Monterrey/projectnotebook june/day06
From 2011.igem.org
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| - | < | + | <p class="justtext"> |
| + | 06/June/2011 | ||
| + | </br> | ||
| + | • miniprep of 22P (103.2 NG/ µ L ) and ( 114 ng / µ L ) | ||
| + | </br> | ||
| + | • Digestions | ||
| + | </br> | ||
| + | • Sterilize material | ||
| + | </br> | ||
| + | • Preparation of Km and Ap stock. | ||
| + | </br> | ||
| + | • Electrophoresis | ||
| + | </br> | ||
| + | • Transformation in the microwave | ||
| + | </br> | ||
| + | • 22 P + 2 M -> in a plate with Ap | ||
| + | </br> | ||
| + | • 16 K + 20 B + 14 N -> in plates with Km | ||
| + | </br> | ||
| + | • We chose one colony of the 22P + 2 M and put it in 10 ml LB media + 100 | ||
| + | µg / mL) | ||
| + | </p> | ||
Revision as of 02:13, 28 September 2011
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MAIN
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PROJECT
06/June/2011 • miniprep of 22P (103.2 NG/ µ L ) and ( 114 ng / µ L ) • Digestions • Sterilize material • Preparation of Km and Ap stock. • Electrophoresis • Transformation in the microwave • 22 P + 2 M -> in a plate with Ap • 16 K + 20 B + 14 N -> in plates with Km • We chose one colony of the 22P + 2 M and put it in 10 ml LB media + 100 µg / mL)
