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Team:WashU/Notebook/Transformation
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LB plates: 150 mL h20 + 6.25 g LB broth + 3.75 g Bacto Agar to Erlenmeyer flask | LB plates: 150 mL h20 + 6.25 g LB broth + 3.75 g Bacto Agar to Erlenmeyer flask | ||
add water to 250 mL (final volume ended up being ~130 mL) | add water to 250 mL (final volume ended up being ~130 mL) | ||
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Chloramphenicol stock solution: 0.07 g chloramphenicol into 2 mL, filter-sterilize; add ~130 uL stock solution into LB (1:1000 dilution) | Chloramphenicol stock solution: 0.07 g chloramphenicol into 2 mL, filter-sterilize; add ~130 uL stock solution into LB (1:1000 dilution) | ||
Revision as of 10:04, 24 September 2011
Transformation Group
The transformation team worked on the bacterial transformation. The team took any DNA plasmid and transforms the plasmid into the bacterial genome. We then cultured the transformed bacteria and isolate the DNA plasmid. This procedure amplifies the amount of DNA plasmid.
The team also transformed DNA plasmid into yeast genome. After the microbiology group ligate the genes in the plasmid, we were responsible for inserting the genes into yeast. We were responsible for sporulation of the yeast with different gene insertions to produce daughter cells with both genes of interest.
Sept. 24
pSB1C3 plasmid arrived; genes ligated into plasmid by MolBio group Transformed E. coli LB plates: 150 mL h20 + 6.25 g LB broth + 3.75 g Bacto Agar to Erlenmeyer flask add water to 250 mL (final volume ended up being ~130 mL)
Chloramphenicol stock solution: 0.07 g chloramphenicol into 2 mL, filter-sterilize; add ~130 uL stock solution into LB (1:1000 dilution)
