Team:ZJU-China

From 2011.igem.org

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                     <p>Being self-organized, highly tolerant and moduled are the main reasons why we chose biofilm as a new synthetic system. Researchers have found that an oxygen gradient is established automatically with the depth of biofilm. Such property allows us to use oxygen sensitive promoters to artificially induce a differentiated expression through the spatial distribution of cells. The natural capability to sustain higher levels of toxin renders biofilm a more stable expression system.
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                     <p>   Rainbofilm is a stratified expression system in biofilm, a self-organized module extensible for various needs. Researchers found a vertical oxygen gradient establishes in the biofilm. Such property allows us to use oxygen sensitive promoters to artificially induce differentiated functions through the spatial distribution of cells. Thus, the multi-step reaction can be processed within the different layers of the biofilm.<br/>
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    The biofilm and its layered structure form spontaneously. Also biofilm has the natural resistance to high levels of toxin. These two properties render the Rainbofilm a convenient stable system for bio-production and bio-sensor. The system can cater to different needs simply by changing downstream genes. One possible application is ethanol production. The cellulose is degraded to monose from the bottom to the middle layer, and the ethanol is produced and secreted in the surface to minimize the toxicity to the inner cells.</p>
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Furthermore, convenience and generality makes our biolfilm system highly applicable. Biofilm is formed automatically, which saves much labor compared with traditional bio-reactors. The reaction system can be easily designed and cater to different needs simply with a change of downstream reporter genes. Through stratified biofilm, the cellulose is degraded to monose from the bottom to the middle layer, and the ethanol is produced in the surface.</p>
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Revision as of 06:31, 25 September 2011

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Welcome


Welcome to the Home of ZJU-CHINA iGEM TEAM. Though it's only a demo version of our wiki, we're hoping it'll be a good start for you to get to know ZJU-CHINA and us. Our team progress and more info will be frequently updated so that our iGEM experience could be shared as much as possible along the way. We truly welcome you to contact us for any cooperation or suggestion.

Project


Rainbofilm is a stratified expression system in biofilm, a self-organized module extensible for various needs. Researchers found a vertical oxygen gradient establishes in the biofilm. Such property allows us to use oxygen sensitive promoters to artificially induce differentiated functions through the spatial distribution of cells. Thus, the multi-step reaction can be processed within the different layers of the biofilm.
The biofilm and its layered structure form spontaneously. Also biofilm has the natural resistance to high levels of toxin. These two properties render the Rainbofilm a convenient stable system for bio-production and bio-sensor. The system can cater to different needs simply by changing downstream genes. One possible application is ethanol production. The cellulose is degraded to monose from the bottom to the middle layer, and the ethanol is produced and secreted in the surface to minimize the toxicity to the inner cells.

Team


We are a young and happy team of 13 scientists and engineers from very diverse backgrounds.Although this is our first year working on a wet lab project and biofilm experiments are new to us, we're currently taking great efforts to build the whole system for experiments.Our team progress and more info will be frequently updated so that our iGEM experience could be shared as much as possible along the way. We truly welcome you to contact us for any cooperation or suggestion.

Gallery


Sponsors


Welcome to the Home of ZJU-CHINA iGEM TEAM. Though it's only a demo version