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Project

このページにはプロジェクトの概要を書いて詳細はリンク先に書くことを提案します。

Overview

Escherichia coli is generally not regarded as an animal because they don't hunt or eat. Thus, the creation of carnivorous E.coli which catches and digests bugs like other animals challenges this notion. Fries are baited, caught and digested, becoming a supplementary ammoniacal source when the media doesn't contain enough gultamine. The project consists of 4 minor projects.

Luminescence: We demonstrated the strong phototaxis to ultraviolet and weaker phototaxis to lights of other wavelength.

Digestion: Chitin, the main component of exoskeleton of fry is degraded by ChitinaseA1 and protein which composes the body is degraded by SAM-P20, serine protease. The exocrine of ChitinaseA1 and SAM-P20 was measured both quantitatively and qualitatively.

Predation: We confirmed that the viscosity of n-acetylglucosamine made by E.coli is enough to trap fries.

Nutritional Signal: Nutritional switch is constructed by the genes from glnA operon.

求訂正。論文用の書き出しなのでwikiではもっと詳しくしないといけない。そもそも実験がすべてうまくいった前提で書いてあることに注意。

Project Details

Project Hunger

Project Luminescence

Introduction made by 草場 ※要添削
In nature, some of organisms which eat insects lure them. Rafflesia attract flies by distinctive smell. Pyrearinus termitilluminans, which makes and lives in tunnel in anthill of termite Cornitermes cumulans, emits light in the first week of rainy season to hunt the termites. Arachnocampa also uses light for hunting flies. For Carnivorous E.coli, we chose light as the method to lure insects like Pyrearinus termitilluminans, because it is simple and there is an available biobrick which has the function to emit light: BBa_K3225909 created by iGEM 2010 Cambridge Team. ===Our works=== To probe that Carnivorous E.coli can lure insects. we, group luminescence, intend to do the following things. *Confirmation that drosophila, our model organism, have positive phototaxis in blue light (E.glowli created by 2010 Cambridge Team emits blue light) *Confirmation that drosophila moves to light emitted by E.coli

Notebook

目標：大腸菌を光らせ、その光にハエを寄せ付ける。

手法：まず、LEDの光に対するハエの走行性を観察。

　　　水平面に垂直に立てた、Ｙ字型のmazeにハエを雄雌別で5匹ずつ3分間走らせた。

　　　LEDはmazeの二つのゴール地点のうち両方に設置した。そして片方だけ灯し、もう片方は無灯火にした状態でハエを投入した。

　　　mazeを水平面に対して垂直にしたのは、ハエの走地性を利用して明確な実験結果を得るためである。

　　　その観察結果から、確かに大腸菌から発せられる495nm付近の波長にハエは引きつけられることが確認できた。

　　　2010年のCambridgeによる研究の手法を使い光る大腸菌を作り、その光にハエをひきつける。

Project Predation

Project Digestion

目標:大腸菌から消化酵素（プロテアーゼ、キチナーゼ）を分泌させ、実際にハエを溶かす。

Introduction

Streptomyces is a kind of prokaryotic bacteria which decompose bodies in nature. We extract protease and chitinase genes from this bacterium and introduce into Escherichia coli. Secretion-signal sequences are included in these genes so that the proteins coded by them will go out without occurring cell lysis. After assembling all genes, we examined the activity of these two enzymes in both of qualitative and quantitative ways.

手法：実験方法は二種類に分けられる。

　　　まず、Streptomyces由来のプロテアーゼを分泌する大腸菌を一定時間液体培養し、その後大腸菌を除去した。

　　　その培地に対して、プロテアーゼアッセイキットを使い培地を光らせ・・・・・