Team:Tec-Monterrey/projectnotebook may/day20

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20/May/2011
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</br>
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• DNA digestión with SspI and NdeI + Buffer 3 at 37 C , 1 hr.
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</br>
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• Electrophoresis 60 V, 1.5 dr.
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</br>
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• Pre- inocolum of virgin cells in 15-20 microliters of LB without antibiotic
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for calcium competent cells.
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</br>
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• Purification of DNA of the agarose gel.
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</p>
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Revision as of 01:53, 28 September 2011

wiki

iGEM

 

 


20/May/2011
• DNA digestión with SspI and NdeI + Buffer 3 at 37 C , 1 hr.
• Electrophoresis 60 V, 1.5 dr.
• Pre- inocolum of virgin cells in 15-20 microliters of LB without antibiotic for calcium competent cells.
• Purification of DNA of the agarose gel.


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