Revision as of 04:59, 23 September 2011

Protocol

[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Medium for drosophila]
Materials	Methods
water : 500mL dry yeast : 20g corn flour : 45g glucose : 50g agarose : 3.5~5g propionic acid : 1.5mL 10% p-hydroxybenzoate in 70% Eternol : 5g	Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it. Stir corn flour and glucose with the remaining water. Stir 1 and 2, then autoclave it again. after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■

Materials	Methods
water : 1 L Na2HPO4: 6 g KH2PO4 : 3 g NaCl : 0.5 g NH4Cl : 1 g 100 mM MgSO4 : 10 ml 20% glucose : 10 mL 10 mM CaCl2 : 10 ml 100 mM thiamine-HCl : 10 ml	Stir Na2HPO4, KH2PO4, NaCl and NH4Cl with water. After autoclave, add 10 ml filter sterilized 100 mM MgSO4, 20% glucose, 10 mM CaCl2, 100 mM thiamine-HCl.

@@ Line 45: / Line 45: @@
 * 100 mM thiamine-HCl : 10 ml
 |
-# Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
+# Stir Na2HPO4, KH2PO4, NaCl and NH4Cl with water.
-# Stir corn flour and glucose with the remaining water.
+# After autoclave, add 10 ml filter sterilized 100 mM MgSO4, 20% glucose, 10 mM CaCl2, 100 mM thiamine-HCl.
-# Stir 1 and 2, then autoclave it again.
-# after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■
 |}
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