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Team:Tec-Monterrey/projectresults
From 2011.igem.org
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| + | <p class="textojustif"> There are several tecniques to prove the successful transportation of CelD and SacC into the outer membrane of <i>E. coli</i>. In this project, SDS-PAGE of entire cell culture samples, SDS-PAGE of membrane fraction samples, and measurement of enzyme activity of whole-cell-system without any chemical purification operation have been considered to confirm the presence of active enzymes on the external membrane. | ||
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Revision as of 10:42, 28 September 2011
There are several tecniques to prove the successful transportation of CelD and SacC into the outer membrane of E. coli. In this project, SDS-PAGE of entire cell culture samples, SDS-PAGE of membrane fraction samples, and measurement of enzyme activity of whole-cell-system without any chemical purification operation have been considered to confirm the presence of active enzymes on the external membrane.
