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Team:Kyoto/Capture
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= '''Project Luminescence''' = | = '''Project Luminescence''' = | ||
The Phototaxis of Drosophila melanogaster against lights of different wavelengths from --nm to --nm and weak luminescence emitted by Luxbrick(BBa_K325909, iGEM Cambridge2010) were evaluated. Drosophila melanogaster was chosen as the object for our research because of its popularity among Japanese houses, facility or easiness to breed and genetical identity which results in the similar behavior. From the result of the research, Drosophila melanogaster was demonstrated to be a good ammoniacal source for genetically modified bacteria. | The Phototaxis of Drosophila melanogaster against lights of different wavelengths from --nm to --nm and weak luminescence emitted by Luxbrick(BBa_K325909, iGEM Cambridge2010) were evaluated. Drosophila melanogaster was chosen as the object for our research because of its popularity among Japanese houses, facility or easiness to breed and genetical identity which results in the similar behavior. From the result of the research, Drosophila melanogaster was demonstrated to be a good ammoniacal source for genetically modified bacteria. | ||
Revision as of 00:35, 23 September 2011
Contents |
Project Luminescence
The Phototaxis of Drosophila melanogaster against lights of different wavelengths from --nm to --nm and weak luminescence emitted by Luxbrick(BBa_K325909, iGEM Cambridge2010) were evaluated. Drosophila melanogaster was chosen as the object for our research because of its popularity among Japanese houses, facility or easiness to breed and genetical identity which results in the similar behavior. From the result of the research, Drosophila melanogaster was demonstrated to be a good ammoniacal source for genetically modified bacteria.
Introduction
Materials and Methods
Apparatus
Y maze
Y maze was constructed by the commonly available straws,__ , LEDs and __ to clamp LEDs. Straws were _色_ striped, in diameter and made of _. _ was made of metal, the angles of Y-form was _ in its top and _ in its side. LEDs were ,, and. __s were cut out of _ into re-entrant shape whose detailed dimension is described below in the figure. After preparing all in above they are connected together with tape and put into the dark room.
ここに装置全体の写真とイラストを載せる。
↑なし/ Diagrammatic representation of the Y maze equipment constructed from straws and __.
ここにLED固定用の装置の寸法を記したイラスト/写真を載せる
Pooter(吸虫管)
Straw was constructed by the commonly available straw, plastic tip and tissue. The tips were used as mouthpiece and objective end.
ここに装置全体の写真とイラスト(完成図と制作方法)を載せる。
↑なし/ Diagrammatic representation of the pooter constructed from a straw, pipets and tissue.
Object
The flies were derived on _日付_ August 2011 from the _strain_ of Drosophila melanogaster kept in _場所_. They were maintained in the temperature controlled incubator at _℃ with a _h light phase(LD 時間:時間) and cultured in the ordinary glass bottle (cm high, cm in diameter)with _培地の名前_medium. The medium was produced according to the method in http://. Every day, the newly born flies were transferred to another bottle to control the postnatal day. The population size of the flies were between _to_.
Method
ハエオス/メス○匹ずつを吸虫管
