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Team:Paris Bettencourt/Experiments/Methodologies/K090403J
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| - | ==Integration== | + | ==Integration plasmid== |
In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team. | In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team. | ||
Revision as of 01:56, 22 September 2011
Integration plasmid
In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
 |
At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (K143001).
After, we want to clone them into the integrative plasmid (K090403).
