Team:Freiburg/Results

From 2011.igem.org

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GFP served as a reporter of expression. We wanted to know how strong the promoter and RBS activity is. With this reporter gene it was possible to analyze the expression via plate reader. GFP is excited at a wavelength of 509nm and has an emission of 520nm. The plate reader illuminates the samples with a high energy xenon flash lamp. Optical filters or monochromator create the exact wavelength. The more GFP in the sample the higher is the GFP fluorescence intensity. The intensity is collected with the second optical system and is detected with a side window photomultiplier tube.
GFP served as a reporter of expression. We wanted to know how strong the promoter and RBS activity is. With this reporter gene it was possible to analyze the expression via plate reader. GFP is excited at a wavelength of 509nm and has an emission of 520nm. The plate reader illuminates the samples with a high energy xenon flash lamp. Optical filters or monochromator create the exact wavelength. The more GFP in the sample the higher is the GFP fluorescence intensity. The intensity is collected with the second optical system and is detected with a side window photomultiplier tube.
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[[File:Freiburg2011_GFP1.jpg|400px|thumbs|GFP fluorescence intensity dependent on the strenght of promoter and RBS.]]
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[[File:Freiburg2011_GFP1.jpg|400px|thumb|GFP fluorescence intensity dependent on the strenght of promoter and RBS.]]
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With RFP (Red Fluorescence Protein) the activity of promoter and RBS can be quantified.
With RFP (Red Fluorescence Protein) the activity of promoter and RBS can be quantified.
RFP served like GFP as a reporter to determine the expression level. RFP is excited at a wavelength of 580nm. The more RFP in the solution the more is the RFP fluorescence intensity.The plate reader illuminates the samples with a high energy xenon flash lamp. Optical filters or monochromator create the exact wavelength. The more RFP in the sample the higher is the RFP fluorescence intensity. The intensity is collected with the second optical system and is detected with a side window photomultiplier tube.
RFP served like GFP as a reporter to determine the expression level. RFP is excited at a wavelength of 580nm. The more RFP in the solution the more is the RFP fluorescence intensity.The plate reader illuminates the samples with a high energy xenon flash lamp. Optical filters or monochromator create the exact wavelength. The more RFP in the sample the higher is the RFP fluorescence intensity. The intensity is collected with the second optical system and is detected with a side window photomultiplier tube.
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[[File:Freiburg2011_RFP.jpg]]
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[[File:Freiburg2011_RFP.jpg|400px|thumb|RFP fluorescence intensity dependent on the strength of promotor and RBS.]]
[http://partsregistry.org/Part:BBa_K608013 BBa_K608013]
[http://partsregistry.org/Part:BBa_K608013 BBa_K608013]

Revision as of 01:54, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!