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Team:Paris Bettencourt/Experiments/Methodologies/K090403J
From 2011.igem.org
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After having used a replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team. | After having used a replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team. | ||
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|+T7 autoloop RFP+ Control / E-coli at 37°C | |+T7 autoloop RFP+ Control / E-coli at 37°C | ||
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| - | |[[File: | + | |[[File:T7 autoloop ctrl-7.jpg|290px|thumb|center|E-Coli at 37°C (trans image)]] |
| + | |[[File:T7 autoloop ctrl-8.jpg|290px|thumb|center|E-Coli at 37°C (gfp image)]] | ||
| + | |[[File:T7 autoloop ctrl-9.jpg|290px|thumb|center|E-Coli at 37°C (rfp image)]] | ||
|- | |- | ||
| + | |[[File:T7 autoloop ctrl-10.jpg|290px|thumb|center|E-Coli at 37°C (trans image)]] | ||
| + | |[[File:T7 autoloop ctrl-11.jpg|290px|thumb|center|E-Coli at 37°C (gfp image)]] | ||
| + | |[[File:T7 autoloop ctrl-12.jpg|290px|thumb|center|-Coli at 37°C (rfp image)]] | ||
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Revision as of 01:36, 22 September 2011
After having used a replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
{| border="1" class="wikitable" style="text-align: center;"
|+T7 autoloop RFP+ Control / E-coli at 37°C
|-
|[[File:T7 autoloop ctrl-7.jpg|290px|thumb|center|E-Coli at 37°C (trans image)]]
|[[File:T7 autoloop ctrl-8.jpg|290px|thumb|center|E-Coli at 37°C (gfp image)]]
|[[File:T7 autoloop ctrl-9.jpg|290px|thumb|center|E-Coli at 37°C (rfp image)]]
|-
|[[File:T7 autoloop ctrl-10.jpg|290px|thumb|center|E-Coli at 37°C (trans image)]]
|[[File:T7 autoloop ctrl-11.jpg|290px|thumb|center|E-Coli at 37°C (gfp image)]]
|[[File:T7 autoloop ctrl-12.jpg|290px|thumb|center|-Coli at 37°C (rfp image)]]
|}
