Team:Wageningen UR/Project/PartsProj1

Data for our favorite new BioBrick parts

[http://partsregistry.org/Part:BBa_K546546 BBa_K546546] - The Synchroscillator: Our goal was to achieve synchronized oscillatory behavior in a population of E. coli cells. Our data shows that this has been achieved. Moreover, we have designed the system in such a way that its expression dynamics are tuneable when operated in conjunction with a tuner device.

[http://partsregistry.org/Part:BBa_K546005 BBa_K546005] - AHL-induced AHL synthase generator with fluorescent reporter: Our data shows an increase in GFP expression per cell over time, providing evidence that the GFP expression is under regulation of a positive feedback loop. This is in accordance with our expectations.

[http://partsregistry.org/Part:BBa_K546001 BBa_K546001] - AHL-induced AHL hydrolase generator: From our data we can conclude that AHL-induced GFP expression significantly drops after treatment with AiiA in contrast to no treatment with AiiA. This is in accordance with our expectations.

Data for pre-existing BioBrick parts

[http://partsregistry.org/Part:BBa_I0460:Experience BBa_I0460] - aiiA Device: Autoinducer inactivation enzyme A (AiiA) is capable of degrading the quorum sensing molecule AHL.

[http://partsregistry.org/Part:BBa_K082014:Experience BBa_K082014] - Autoinducer synthethase: Produces the quorum sensing molecule 3-oxohexanoyl-homoserine lactone (AHL).

[http://partsregistry.org/Part:BBa_I763020:Experience BBa_I763020] - GFP + LVA Tag This GFP with an LVA-tag functioned as expected: our data clearly shows that the GFP is rapidly being degraded.

[http://partsregistry.org/Part:BBa_F2621:Experience BBa_F2621] - F2621 Our sequencing data showed that this part was incomplete and therefore we repaired it and also experimentally proved that our repaired part had the functionality the received part was lacking.

[http://partsregistry.org/Part:BBa_J24675:Experience BBa_J24675] - J24675 We placed a promoter in front of this part and then sent the hereby created part for sequencing. The sequence data did not correspond to the sequence obtained from the Registry of Standard Biological Parts.

Other new BioBrickparts

[http://partsregistry.org/Part:BBa_K546002 BBa_K546002] - AHL-induced GFP reporter Our data shows that upon addition of AHL the expression of GFP is increased. This BioBrick part thus works as expected.

[http://partsregistry.org/Part:BBa_K546000 BBa_K546000] - AHL-induced Ahl synthase generator: Our data shows an increase in GFP expression per cell over time, providing evidence that the GFP expression is under regulation of a positive feedback loop. This is in accordance with our expectations. This BioBrick part does not actually contain GFP(BBa_K546002), but it can be deduced to work the same as BBa_546005.

[http://partsregistry.org/Part:BBa_K546003 BBa_K546003] - Fixed F2621 Our data shows that by adding the R0063 promoter to the broken F2621 we restored the BioBrick part to proper functionality. The reason for malfunctioning is that if LuxR isn't present because of the absence of the promoter, no LuxR-AHL complex can be formed and therefore the downstream pathway doesn't react to an increasing AHL concentration anymore.