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Team:Paris Bettencourt/Designs/List
From 2011.igem.org
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<h1>Design List</h1> | <h1>Design List</h1> | ||
| - | <h2> | + | <h2>Concentrator</h2> |
| - | <p> | + | <p>In order to observe a celar change in phenotype during diffusion experiments, we worked on a YFP-TetR fusion protein concentrator.</p> |
<table> | <table> | ||
<tr> | <tr> | ||
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</td> | </td> | ||
</tr> | </tr> | ||
| + | </table> | ||
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| + | <h2>Positive feedback autoloop</h2> | ||
| + | <p>We designed entirely these new devices. They are composed of an emitter, a receptor and an amplifier sub-unit.</p> | ||
| + | <table> | ||
<tr> | <tr> | ||
<td style="width:200px; text-align:center;"><a href="https://2011.igem.org/Team:Paris_Bettencourt/T7_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/e/e4/T7_button.png"></a> | <td style="width:200px; text-align:center;"><a href="https://2011.igem.org/Team:Paris_Bettencourt/T7_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/e/e4/T7_button.png"></a> | ||
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<td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/tRNA_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/5/53/TRNAamber-button.png"></a> | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/tRNA_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/5/53/TRNAamber-button.png"></a> | ||
</td> | </td> | ||
| - | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/tRNA_diffusion">tRNA amber diffusion</a></b> The tRNA amber | + | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/tRNA_diffusion">tRNA amber diffusion</a></b> The tRNA amber allows the translation of a functionnal T7 RNA polymerase in the receiver cell. This will then trigger the auto-amplification loop. |
</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
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<h2>Using bistable switches</h2> | <h2>Using bistable switches</h2> | ||
<p>During our brainstormings, we noticed several natural or artificial bistable switches that could serve both as a receptor and an auto-amplifier. One molecule carefully chosen could toggle the switch in another position. All we have to do is see if it diffuses through the nanotubes. | <p>During our brainstormings, we noticed several natural or artificial bistable switches that could serve both as a receptor and an auto-amplifier. One molecule carefully chosen could toggle the switch in another position. All we have to do is see if it diffuses through the nanotubes. | ||
Revision as of 12:24, 11 October 2011
Design List
Concentrator
In order to observe a celar change in phenotype during diffusion experiments, we worked on a YFP-TetR fusion protein concentrator.
|
YFP concentration This design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins. |
Positive feedback autoloop
We designed entirely these new devices. They are composed of an emitter, a receptor and an amplifier sub-unit.
|
T7 RNA polymerase diffusion In this design, we introduce the use of the T7 polymerase both as the transfer molecule and as the auto-amplification system. |
|
tRNA amber diffusion The tRNA amber allows the translation of a functionnal T7 RNA polymerase in the receiver cell. This will then trigger the auto-amplification loop. |
Using bistable switches
During our brainstormings, we noticed several natural or artificial bistable switches that could serve both as a receptor and an auto-amplifier. One molecule carefully chosen could toggle the switch in another position. All we have to do is see if it diffuses through the nanotubes.
|
ComS diffusion We took advantage of a switch already existing in B.Subtilis (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes. |
|
Sin Operon |
Lambda switch
|
Lambda switch |
