"
Team:UCL London/Research/Supercoilometer/Experiments
From 2011.igem.org
(Difference between revisions)
| Line 11: | Line 11: | ||
<h2>Parts used</h2> | <h2>Parts used</h2> | ||
| - | <html><table border=" | + | <html><table border="1" cellspacing="0" cellpadding="0"> |
<tr> | <tr> | ||
<td width="91" valign="top" bgcolor="#CCCCCC"><p><strong>Part Name</strong></p></td> | <td width="91" valign="top" bgcolor="#CCCCCC"><p><strong>Part Name</strong></p></td> | ||
<td width="82" valign="top" bgcolor="#CCCCCC"><p align="center"><strong>Parts ID</strong></p></td> | <td width="82" valign="top" bgcolor="#CCCCCC"><p align="center"><strong>Parts ID</strong></p></td> | ||
<td width="146" valign="top" bgcolor="#CCCCCC"><p align="center"><strong>Parts Description</strong></p></td> | <td width="146" valign="top" bgcolor="#CCCCCC"><p align="center"><strong>Parts Description</strong></p></td> | ||
| - | <td width=" | + | <td width="120" valign="top" bgcolor="#CCCCCC"><p align="center"><strong>Length (bp)</strong></p></td> |
| - | <td width=" | + | <td width="200" valign="top" bgcolor="#CCCCCC"><p align="center"><strong>Plasmid Backbone</strong></p></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 33: | Line 33: | ||
<td width="109" valign="top"><p>n/a</p></td> | <td width="109" valign="top"><p>n/a</p></td> | ||
</tr> | </tr> | ||
| - | </table | + | </table> |
| - | + | ||
{{:Team:UCL_London/Template/Footer}} | {{:Team:UCL_London/Template/Footer}} | ||
Revision as of 23:28, 21 September 2011
Building a Supercoilometer in the lab
Aim
To prepare a BioBrick from the tyrT promoter cloned from the E. coli genome and then construct and characterise a device which can detect the level of negative supercoiling in a closed circular plasmid.
Parts used
Part Name |
Parts ID |
Parts Description |
Length (bp) |
Plasmid Backbone |
RBS+CFP.LVA+Terminator |
RBS + cyan fluorescent protein with LVA tag + terminator |
917 |
on pSB1A2 plasmid (2079 bp) - ampicillin resistance |
|
n/a |
standard plasmid backbone (chloramphenicol resistance) |
2070 |
n/a |
