Team:Potsdam Bioware/Data Page

DataPage

Data For Our New Favorite BioBricks

1. [http://partsregistry.org/Part:BBa_K627006 Main Page] - Fusion part of mdnA gene with c-terminal myc-tag and gene III, BBa_K627006: This BioBrick is an important milestone in the development of a functional phage display system for screening of microviridin, which are encoded by the mdnA gene libraries. [More Wiki]

2. [http://partsregistry.org/Part:BBa_K627012 Main Page] - Fusion of ssTorA, TEV cleavage site and b-lactamase (full length), BBa_K627012: The BioBrick BBa_K627012 consists of the cleavage site for the TEV protease, which is flanked by the sequence of the beta-lactamse and of the TorA signal. Thus the beta-lactamase can be transported into the periplasm and can provide resistance against beta-lactam antibiotics, such as ampicillin or penicillin. [More Wiki]

3. [http://partsregistry.org/Part:BBa_K627011 Main Page] - Fusion of AraC induction system and 14_3C protease, BBa_K627011: In case of expression of the BioBrick BBa_K267011 beta lactamase can be transported in periplasm and can provide resistance against beta-lactam antibiotics, such as ampicillin and penicillin. [More Wiki]

4. [http://partsregistry.org/Part:BBa_K627001 Main Page] - Gene mdnA encoding the ribosomal precursor peptide of the mdn-cluster, BBa_K627001: The BioBrick BBa_K627001 encodes the ribosomal precursor peptide, which is essential for the production of tricyclic protease inhibitor microviridin. [More Wiki]

Data For Pre-existing Parts

1. [http://partsregistry.org/Part:BBa_I757010:Experience Experience] - b-lactamase TEM optimized, BBa_I757010: The b-lactamase providing resistance against the antibiotic ampicillin was used in combination with signal sequence TorA. This fusion part displays a novel protease activity detector device, which shows an increased survival rate at 30°C and 37°C.

2. [http://partsregistry.org/Part:BBa_K208005:Experience Experience] - TorA Signal Peptide - Silver Fusion Compatible, BBa_K627005: The Silver-fusion compatible BioBrick BBa_K208005 is improved by attaching a cleavage site for different proteases and the b-lactamase providing resistance against the antibiotic ampicillin. A high export rate via the TAT pathway was proven.

We've Also Characterized the Following Parts

1. [http://partsregistry.org/Part:BBa_K627000 Main Page] - ABC transporter and N-acetyltransferase from mdn-cluster, BBa_K627000: The BioBrick BBa_K627000 is a part of the microviridin gene (mdn) cluster, which encodes the protease inhibitor microviridin L. The N-acetyltransferase and the ABC transporter encoded by the BioBrick BBa_K627000 are involved in the scaffold and cyclization of the microviridin peptide. [More Wiki]

2. [http://partsregistry.org/Part:BBa_K627002 Main Page] - ATP-grasp-type ligase from mdn-cluster, BBa_K627002: The BioBrick BBa_K627002 is a part of the microviridin gene (mdn) cluster, which encodes the protease inhibitor microviridin L. The ATP-grasp-type ligase encoded by the BioBrick BBa_K627002 is involved in the scaffold and cyclization of the microviridin peptide. [More Wiki]

3. [http://partsregistry.org/Part:BBa_K627003 Main Page] - ATP-grasp-type ligase from mdn-cluster, BBa_K627003: The BioBrick BBa_K627003 is a part of the microviridin gene (mdn) cluster, which encodes the protease inhibitor microviridin L. The ATP-grasp-type ligase encoded by the BioBrick BBa_K627003 is involved in the scaffold and cyclization of the microviridin peptide. [More Wiki]

4. [http://partsregistry.org/Part:BBa_K627004 Main Page] - N-acetyltransferase from the mdn-cluster, BBa_K627004: The BioBrick BBa_K627004 is a part of the microviridin gene (mdn) cluster, which encodes the protease inhibitor microviridin L. The N-acetyltransferase encoded by the BioBrick BBa_K627004 is involved in the scaffold and cyclization of the microviridin peptide. [More Wiki]

5. [http://partsregistry.org/Part:BBa_K627005 Main Page] - ABC transporter from the mdn-cluster, BBa_K627005: The BioBrick BBa_K627005 is a part of the microviridin gene (mdn) cluster, which encodes the protease inhibitor microviridin L. The ABC transporter encoded by the BioBrick BBa_K627005 is involved in the scaffold and cyclization of the microviridin peptide. [More Wiki]

6. [http://partsregistry.org/Part:BBa_K627008 Main Page] - Fusion of AraC induction system and TEV protease, BBa_K627008: As a fusion of pBAD arabinose-inducible induction system and the TEV protease, this BioBrick can be used for cleaving fusion proteins. This TEV protease variant is resistant to many commonly used protease inhibitors. For this BioBrick equivalent parts exist in the registry, [http://partsregistry.org/Part:BBa_K627009 BBa_K627009], [http://partsregistry.org/Part:BBa_K627010 BBa_K627010] [More Wiki]