Commons
Minipreps
Investigators: Sandra
Because of the positive testdigest, a miniprep of the PR+GFP was performed. Samples were send for sequencing.
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
Miniprep of PR+lovtap-notgate domain
Investigators: Sandra
Miniprep of:
- 39A
- 39B
- 40A
- 40B
- 41A
- 41B
- 42A
- 42B
- 44A
- 44B
39=PR1,
40=PR2,
41=PR3,
42=PR4,
44=PR6.
2A-assembly: Digestion
Investigators: Sandra
Digestion of the samples from miniprep (10 samples) and digestion of RFP (S1b, S2b).
PR with Lov-Notgate domain:
- 39A
- 39B
- 40A
- 40B
- 41A
- 41B
- 42A
- 42B
- 44A
- 44B
digested with NehI and PstI.
RFP:
digested with SpeI and PstI.
2A-assembly: Ligation
Ligation of RFP with 39-44.
Trafo
Trafo of the ligated samples on plates with Cm.
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
High throughput sequencing
Investigators:Theo
All PR parts in C3 vectors, M48 in C3 and also the M48+1 and M48+7 in Amp vectors were sent for sequencing
Precipitator
Testdigest
| Name:Rüdiger
| Date: 12.09.
|
| Continue from Experiment Miniprep (Date) 10.&11.09.
(Name) Rüdiger
|
| Project Name: Precipitator
|
For one reaction you need: For Mastermix: Number of samples+2extra
| 4μl
| H2O
| 280
|
|
| 1μl
| Buffer, NEB4
| 70
|
|
| 1μl
| BSA (10x)
| 70
|
|
| 0,5 μl
| Enzym 1
| 35
| Eco R1
|
| 0,5 μl
| Enzym 2
|
|
|
| 3 μl
| DNA
|
|
|
10 μl total volume
Give 3 μl of DNA in an eppi and add 7μl of the mastermix.
Incubate for about 1h at 37°C.
Add 1 μl Loading dye buffer and load the gel.
Take a picture of the gel, print picture and label the lanes!
| 1aA
| 1aB
| 1aC
| 1bA
| 1bB
| 1bC
| 2aA
| 2aB
| 2aC
|
| Eco R1 + Bam H1
|
| X
| X
| X
| X
| X
| X
| X
| X
| X
|
| 2bA
| 2bB
| 2bC
| 3bA
| 3bB
| 3bC
| 3aA
| 3aB
| 3aC
|
| Eco R1 + Bam H1
|
| X
| X
| X
| X
| X
| X
| X
| X
| X
|
| 6aA
| 6aB
| 6aC
| 74aA
| 7aB
| 7aC
| 7bA
| 7bB
| 7bC
|
| Eco R1 + Pst 1
|
| X
| X
| X
| X
| X
| X
| X
| X
| X
|
| 5bA
| 5bB
| 5bC
| 5aA
| 5aB
| 5aC
| 6bA
| 6bB
| 6bC
|
| Eco R1 + Pst 1
|
| X
| X
| X
| X
| X
| X
| X
| X
| X
|
| 8a1
| 8a3
| 8a4
| 8b1
| 8b2
| 8b4
| 10a2
| 10a3
| 10a4
|
| Eco R1 + Spc 1
|
| X
| X
| X
| X
| X
| X
| X
| X
| X
|
| 9a1
| 9a2
| 9a3
| 9a4
| 9b1
| 9b2
| 9b3
| 9b4
|
| Eco R1 + Spc 1
|
| X
| X
| X
| X
| V
| X
| X
| V
|
| 10b3
| 10b4
| 10b01
| 10b2
| 4a1
| 4a2
| 4a3
| 4b4
|
| Eco R1 + Spc 1
|
| V
| V
| X
| V
| X
| X
| V
| V
|
X=Failure, V=Success
NAME OF YOUR EXPERIMENT
Investigators: NAME
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