Team:Bilkent UNAM Turkey/Experiment

(Difference between revisions)

Revision as of 20:36, 21 September 2011

We ordered our genes. We optimized codon usage because of gene taken from E. cloacae.

Codon Usage Then we did restriction digestion and gel electrophoresis.
We repeat this step a lot because of failuire.I put right result

We used standard gel purification kit Our ligation style is really similar to iGEM's one.
Our one;

insert volume(µl):X

Cut Vector Volume(µl):X

DEPC-water(µl):8,5-2X

T4 DNA ligase(µl):0,5

Ligation Buffer(µl):1

Total Volume(µl):10

We transform our plasmid into E.coli with a protocol.
We used invitrogen purification kit.

@@ Line 5: / Line 5: @@
 We repeat this step a lot because of failuire.I put right result<br>
 <img src="https://static.igem.org/mediawiki/2011/3/3e/Restriction_products_of_minipreps_of_pRbcnfsI_unam.jpg" align="center"></img>
-<br>We used standard <a href="http://tools.invitrogen.com/content/sfs/manuals/purelink_pro96_plasmid_kit_man.pdf">gel purification kit</a>
+<br>We used standard <a href="http://tools.invitrogen.com/content/sfs/manuals/purelink_quick_gel_extraction_kit_man.pdf">gel purification kit</a>
 Our ligation style is really similar to iGEM's one.<br>
 Our one;
@@ Line 14: / Line 14: @@
 <li>Ligation Buffer(µl):1</li>
 <li>Total Volume(µl):10</li>
-<br>We transform our plasmid into E.coli with a <a href="http://www.med.nyu.edu/medicine/labs/blaserlab/Protocols/E-coli_competent_cells_protocol_&_transformation.pdf">protocol</a>
+<br>We transform our plasmid into E.coli with a <a href="http://www.med.nyu.edu/medicine/labs/blaserlab/Protocols/E-coli_competent_cells_protocol_&_transformation.pdf">protocol.</a><br>
+We used invitrogen <a href="http://tools.invitrogen.com/content/sfs/manuals/purelink_pro96_plasmid_kit_man.pdf">purification kit.</a>
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