"
Team:Warsaw/RBSmeasuremen/Results
From 2011.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
<div class="note">RBS Measurement Results</div><br /> | <div class="note">RBS Measurement Results</div><br /> | ||
| - | <div>As you can see below there are significant differences in RBS strength relative to B0034 between various fluorescent proteins. This is probably caused by influence of protein coding sequence on mRNA fold so different proteins will always have different expression levels from the same RBS and promoter. Differences reach 60% so you cannot predict expression level of your favorite protein basing on measurements with GFP.<br> In order to enshure that every protein will behave the same way with given expression driving part we need new approach. We need to deal with mRNA fold so we also need to get rid of different protein beginnings. One of solutions are our <a href="https://2011.igem.org/Team:Warsaw/ExpressionAdaptors">expression adaptors.</a> | + | <div align="justify">As you can see below there are significant differences in RBS strength relative to B0034 between various fluorescent proteins. This is probably caused by influence of protein coding sequence on mRNA fold so different proteins will always have different expression levels from the same RBS and promoter. Differences reach 60% so you cannot predict expression level of your favorite protein basing on measurements with GFP.<br> In order to enshure that every protein will behave the same way with given expression driving part we need new approach. We need to deal with mRNA fold so we also need to get rid of different protein beginnings. One of solutions are our <a href="https://2011.igem.org/Team:Warsaw/ExpressionAdaptors">expression adaptors.</a> |
<br> | <br> | ||
The other solution would be to predict expression strength basing on existing measurements.<ul> | The other solution would be to predict expression strength basing on existing measurements.<ul> | ||
Revision as of 20:19, 21 September 2011
RBS Measurement Results
As you can see below there are significant differences in RBS strength relative to B0034 between various fluorescent proteins. This is probably caused by influence of protein coding sequence on mRNA fold so different proteins will always have different expression levels from the same RBS and promoter. Differences reach 60% so you cannot predict expression level of your favorite protein basing on measurements with GFP.
In order to enshure that every protein will behave the same way with given expression driving part we need new approach. We need to deal with mRNA fold so we also need to get rid of different protein beginnings. One of solutions are our expression adaptors.
The other solution would be to predict expression strength basing on existing measurements.
In order to enshure that every protein will behave the same way with given expression driving part we need new approach. We need to deal with mRNA fold so we also need to get rid of different protein beginnings. One of solutions are our expression adaptors.
The other solution would be to predict expression strength basing on existing measurements.
- It would require creaction of a database of measurements of each RBS with various proteins.
- The protein N terminal parts (e.g. first 10 aa) could be blast against database of N terminal sequences of proteins from the database of measurements of each RBS with various proteins
- The best blast hit could be used to predict expression strength along with computational supporting tools e.g. RBS designer or RBS calculator Unfortunately creating such database is currently not possible during summer project. So far we could tray predicting expression of proteins with similar N-termila sequence to measured 5 fluorescent proteins.
Graphical representation of measurement results
XFP expression driven by different RBS parts in numbers
