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Team:Grenoble/Notebook/September
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Ligation of rposLS amplicons into PSB1C3 and transformation. Screen by PCR, restriction of four clones and sequencing. Construction with fhaLS from pTOPO (EcoRI and PstI) and I13401 (XbaI and EcoRI) and construction with rsmA PCR products. R0011-rsmA-pSB1A2 (construction 31).With R0011 digested by SpeI and PstI and R0011digested by XbaI and PstI. | Ligation of rposLS amplicons into PSB1C3 and transformation. Screen by PCR, restriction of four clones and sequencing. Construction with fhaLS from pTOPO (EcoRI and PstI) and I13401 (XbaI and EcoRI) and construction with rsmA PCR products. R0011-rsmA-pSB1A2 (construction 31).With R0011 digested by SpeI and PstI and R0011digested by XbaI and PstI. | ||
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| - | + | fhaSL-I13401-pSB1AT2 (construction 32) Four positives clones per construction were selected after colony PCR and sent for sequencing. (No restriction screening was performed in order to gain times) | |
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Revision as of 20:10, 21 September 2011
August 4th to 10th
BiologyFélix
Ligation of rposLS amplicons into PSB1C3 and transformation. Screen by PCR, restriction of four clones and sequencing. Construction with fhaLS from pTOPO (EcoRI and PstI) and I13401 (XbaI and EcoRI) and construction with rsmA PCR products. R0011-rsmA-pSB1A2 (construction 31).With R0011 digested by SpeI and PstI and R0011digested by XbaI and PstI.
fhaSL-I13401-pSB1AT2 (construction 32) Four positives clones per construction were selected after colony PCR and sent for sequencing. (No restriction screening was performed in order to gain times)
