Team:KULeuven/Biobricks

From 2011.igem.org

(Difference between revisions)

Revision as of 17:48, 21 September 2011

KULeuven iGEM 2011

Biobricks

The biobricks that we used are on the iGEM registry which can be found here. Underneath you can see several short videos in which we added different kinds of E.D Frosti to supercooled (-6°C) ultrapure “milli-Q” water (Millipore Corporation). The first video (GFP-control), is a control: E.D. Frosti only contains a GFP construct. When we add it to the supercooled water, it does not freeze.

The second video (INP), E.D. Frosti contains a constitutively active INP producing biobrick ( BBa_K584027). When we added this to the supercooled water, it froze immediately.

@@ Line 124: / Line 124: @@
 <br><br>
+The second video (INP), <i> E.D. Frosti</i> contains a constitutively active INP producing biobrick (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584027" target="blank">  <b>BBa_K584027</b></a>).
-In the second video (IPTG), <i>E.D. Frosti</i> contains <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584028" target="blank"> <b>BBa_K584028</b> </a>
-which contains the <i>INP</i> gene, coupled to the lactose inducible promoter. We added 1mM IPTG to <i>E.D. Frosti</i>. When we added it to supercooled water, it froze immediately,
-prooving the functionallity of our biobrick. <br><br>
-In the third video (INP), <i> E.D. Frosti</i> contains a constitutively active INP producing biobrick (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584027" target="blank">  <b>BBa_K584027</b></a>).
 When we added this to the supercooled water, it froze immediately.
 <br><br>
@@ Line 138: / Line 133: @@
 </center>
-The fourth video (No IPTG) shows what happened when we added <i>E.D. Frosti </i> with the <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584028" target="blank"> <b>BBa_K584028</b> </a>
-biobrick, but that did not receive a lactose (IPTG) stimulus. Although our GFP-characterization of this biobrick showed that the promoter was not leaky, the water still froze.
-This can have several causes, two examples are: the coupling of the <i>INP</i> gene to the promoter made the system leaky. It is also possible that there is a very low (basal) expression of the <i>INP</i> gene and that this small amount of INP is enough to induce ice nucleation.
 <br><br>