"
Team:UPO-Sevilla/Project/Improving Flip Flop/Data Page
From 2011.igem.org
| Line 78: | Line 78: | ||
display: inline-block; | display: inline-block; | ||
height: 4em; | height: 4em; | ||
| - | left: - | + | left: -14.5em; |
position: relative; | position: relative; | ||
| - | top: | + | top: 24.5em; |
width: 6.7em;} | width: 6.7em;} | ||
.tour_IPTG{ | .tour_IPTG{ | ||
| Line 86: | Line 86: | ||
display: inline-block; | display: inline-block; | ||
height: 4em; | height: 4em; | ||
| - | left: | + | left: 3.5em; |
position: relative; | position: relative; | ||
| - | top: | + | top: 7em; |
width: 4.7em;} | width: 4.7em;} | ||
</style> | </style> | ||
| Line 182: | Line 182: | ||
"color" : "black", | "color" : "black", | ||
"text" : "When we give an IPTG pulse to our bacteria Lac promoter (PLac) is activated, expressing cI (temperature-sensitive), OmpN::SspB and OmpN::RFP proteins. Now we are in State 2. ", | "text" : "When we give an IPTG pulse to our bacteria Lac promoter (PLac) is activated, expressing cI (temperature-sensitive), OmpN::SspB and OmpN::RFP proteins. Now we are in State 2. ", | ||
| - | "position" : " | + | "position" : "B", |
"time" : 5000 | "time" : 5000 | ||
}, | }, | ||
| Line 190: | Line 190: | ||
"color" : "black", | "color" : "black", | ||
"text" : "cI is cI promoter repressor. This protein represses PcI and, therefore, State 1. This will assure de maintenance of State 2 over State 1 in absence of State 1 stimulus. As this cI is temperature sensitive it is degraded when we give our bacteria the heat shock necessary for State 1 activation.", | "text" : "cI is cI promoter repressor. This protein represses PcI and, therefore, State 1. This will assure de maintenance of State 2 over State 1 in absence of State 1 stimulus. As this cI is temperature sensitive it is degraded when we give our bacteria the heat shock necessary for State 1 activation.", | ||
| - | "position" : " | + | "position" : "B", |
"time" : 5000 | "time" : 5000 | ||
}, | }, | ||
| Line 198: | Line 198: | ||
"color" : "black", | "color" : "black", | ||
"text" : "OmpN::RFP is State 2 reporter, which makes our bacteria glow in red when exposed to UV.", | "text" : "OmpN::RFP is State 2 reporter, which makes our bacteria glow in red when exposed to UV.", | ||
| - | "position" : " | + | "position" : "B", |
"time" : 5000 | "time" : 5000 | ||
}, | }, | ||
| Line 206: | Line 206: | ||
"color" : "black", | "color" : "black", | ||
"text" : "SspB is the adaptor protein that allows the ClpXP protease to degrade DAS+4 tagged proteins, in this case LacI::DAS+4 and GFP::DAS+4. Therefore, State 2 expression also eliminates State 1 reporter and State 2 repressor. ", | "text" : "SspB is the adaptor protein that allows the ClpXP protease to degrade DAS+4 tagged proteins, in this case LacI::DAS+4 and GFP::DAS+4. Therefore, State 2 expression also eliminates State 1 reporter and State 2 repressor. ", | ||
| - | "position" : " | + | "position" : "B", |
"time" : 5000 | "time" : 5000 | ||
}, | }, | ||
| Line 214: | Line 214: | ||
"color" : "black", | "color" : "black", | ||
"text" : "This State won’t change again until we give our bacterias the heat shock.", | "text" : "This State won’t change again until we give our bacterias the heat shock.", | ||
| - | "position" : " | + | "position" : "B", |
"time" : 5000 | "time" : 5000 | ||
}, | }, | ||
Revision as of 16:52, 21 September 2011
Sponsors
