Team:Freiburg/Notebook/3 September

From 2011.igem.org

(Difference between revisions)
(Testdigestion of 2a-assembly)
(3A-assembly:Digestion)
Line 37: Line 37:
'''InsertI:'''
'''InsertI:'''
-
*64
+
*64, CcaR
-
*46  
+
*46, ho1
with EcorI and SpeI
with EcorI and SpeI
'''InsertII:'''
'''InsertII:'''
-
*67
+
*67, CcaS
-
*50
+
*50, pcyA
with XbaI and PstI
with XbaI and PstI
-
Incubation for 6 hours at 37°C. Vector pSB1K3 was already digested, it only needs to be digested with antarctica for one hour.
+
For this assembly we used the constructs from the 2A-assembly made on [https://2011.igem.org/Team:Freiburg/Notebook/31_August 31.08]
 +
 
 +
Incubation for 6 hours at 37°C. Vector pSB1C3 was already digested,<br/>  it was just treated with antarctic phosphatase for one hour.
 +
 
===3A-assembly:Ligation===
===3A-assembly:Ligation===

Revision as of 11:16, 21 September 2011


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