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Team:Penn/Notebook
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'''09/20/2011 (Avin, Mike M, Peter, Anthony)''' | '''09/20/2011 (Avin, Mike M, Peter, Anthony)''' | ||
| - | First time doing calcium imaging. Brought over 293T's in a 6 well plate, transfected with CatCh. Also had positive control cells on which we used ionophores. Used the Meaney Lab confocal and x-rhod1 dye. Media had 2mM Calcium. | + | First time doing calcium imaging. Brought over 293T's in a 6 well plate, which had been transfected with CatCh. Also had positive control cells on which we used ionophores. Used the Meaney Lab confocal and x-rhod1 dye. Media had 2mM Calcium. |
1uM Ionomycin addition did not change x-rhod1 fluorescent intensity in Anthony's neurons. Also did not work on our 293T's but we had the problem of being unable to cleanly pipet the ionomycin into the 6 well plates because the wells we looked at became entirely covered by the scope lens. | 1uM Ionomycin addition did not change x-rhod1 fluorescent intensity in Anthony's neurons. Also did not work on our 293T's but we had the problem of being unable to cleanly pipet the ionomycin into the 6 well plates because the wells we looked at became entirely covered by the scope lens. | ||
Illumination of CatCh cells with 476nm laser from confocal did not increase x-rhod1 intensity. eYFP expressing cells were identified, so transfection worked. Will try again with larger plates, and possibly switch to FURA-2 and Avin's laser if the confocal doesn't work. | Illumination of CatCh cells with 476nm laser from confocal did not increase x-rhod1 intensity. eYFP expressing cells were identified, so transfection worked. Will try again with larger plates, and possibly switch to FURA-2 and Avin's laser if the confocal doesn't work. | ||
Revision as of 03:22, 21 September 2011
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Notebook
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
09/20/2011 (Avin, Mike M, Peter, Anthony)
First time doing calcium imaging. Brought over 293T's in a 6 well plate, which had been transfected with CatCh. Also had positive control cells on which we used ionophores. Used the Meaney Lab confocal and x-rhod1 dye. Media had 2mM Calcium.
1uM Ionomycin addition did not change x-rhod1 fluorescent intensity in Anthony's neurons. Also did not work on our 293T's but we had the problem of being unable to cleanly pipet the ionomycin into the 6 well plates because the wells we looked at became entirely covered by the scope lens.
Illumination of CatCh cells with 476nm laser from confocal did not increase x-rhod1 intensity. eYFP expressing cells were identified, so transfection worked. Will try again with larger plates, and possibly switch to FURA-2 and Avin's laser if the confocal doesn't work.
