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Team:Warsaw/RBSmeasuremen/Results
From 2011.igem.org
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<div class="note">RBS Measurement Results</div><br /> | <div class="note">RBS Measurement Results</div><br /> | ||
| - | <div>As you can see below there are significant differences in RBS strength relative to B0034 between various fluorescent proteins. This is probably caused by influence of protein coding sequence on mRNA fold so different proteins will always have different expression levels from the same RBS and promoter. Differences reach 60% so you cannot predict expression level of your favorite protein basing on measurements with GFP.<br> In order to enshure that every protein will behave the same way with given expression driving part we need new approach. We need to deal with mRNA fold so we also need to get rid of different protein beginnings. One of | + | <div>As you can see below there are significant differences in RBS strength relative to B0034 between various fluorescent proteins. This is probably caused by influence of protein coding sequence on mRNA fold so different proteins will always have different expression levels from the same RBS and promoter. Differences reach 60% so you cannot predict expression level of your favorite protein basing on measurements with GFP.<br> In order to enshure that every protein will behave the same way with given expression driving part we need new approach. We need to deal with mRNA fold so we also need to get rid of different protein beginnings. One of solutions are our <a href="https://2011.igem.org/Team:Warsaw/ExpressionAdaptors">expression adaptors</a></div> |
Revision as of 23:46, 20 September 2011
RBS Measurement Results
As you can see below there are significant differences in RBS strength relative to B0034 between various fluorescent proteins. This is probably caused by influence of protein coding sequence on mRNA fold so different proteins will always have different expression levels from the same RBS and promoter. Differences reach 60% so you cannot predict expression level of your favorite protein basing on measurements with GFP.
In order to enshure that every protein will behave the same way with given expression driving part we need new approach. We need to deal with mRNA fold so we also need to get rid of different protein beginnings. One of solutions are our expression adaptors
In order to enshure that every protein will behave the same way with given expression driving part we need new approach. We need to deal with mRNA fold so we also need to get rid of different protein beginnings. One of solutions are our expression adaptors
Graphical representation of measurement results
XFP expressio driven by different RBS parts in numbers
