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Team:Freiburg/Results
From 2011.igem.org
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[http://partsregistry.org/Part:BBa_K608406 BBa_K608406] | [http://partsregistry.org/Part:BBa_K608406 BBa_K608406] | ||
| - | Precipitator | + | '''Precipitator |
| - | + | ''' | |
The Precipitator is a new artificially designed LRR protein. It is meant as protein that binds Nickel ions with Histidines grouped on its surface. The bound Nickel can then precipitate His-tagged proteins. In our Lab in a Cell it should function as an adaptor between the plastic surface of pipettes and the His-tagged protein. Please look at our detailed description of the design layout in our modeling section. The sequence was synthesised and cloned into the iGEm vector. The sequence was partially confirmed by sequencing. | The Precipitator is a new artificially designed LRR protein. It is meant as protein that binds Nickel ions with Histidines grouped on its surface. The bound Nickel can then precipitate His-tagged proteins. In our Lab in a Cell it should function as an adaptor between the plastic surface of pipettes and the His-tagged protein. Please look at our detailed description of the design layout in our modeling section. The sequence was synthesised and cloned into the iGEm vector. The sequence was partially confirmed by sequencing. | ||
[http://partsregistry.org/Part:BBa_K608407 BBa_K608407] | [http://partsregistry.org/Part:BBa_K608407 BBa_K608407] | ||
| - | Precipitator fused with GST tag | + | '''Precipitator fused with GST tag''' |
The Precipitator was fused with the C-terminus of our GST tag in order to extract and further test the construct for its Nickel binding affinity. We successfully cloned it together using the Gibson Assembly and then subsequently pasted it into the iGEM vector. The sequence was partially confirmed by sequencing. | The Precipitator was fused with the C-terminus of our GST tag in order to extract and further test the construct for its Nickel binding affinity. We successfully cloned it together using the Gibson Assembly and then subsequently pasted it into the iGEM vector. The sequence was partially confirmed by sequencing. | ||
[http://partsregistry.org/Part:BBa_K608408 BBa_K608408] | [http://partsregistry.org/Part:BBa_K608408 BBa_K608408] | ||
| - | GST-tag | + | '''GST-tag''' |
The GST-tag was PCR amplified from a pGEX vector with overhang primers including the iGEM restriction sites and then pasted into the iGEM vector. To verify the functionality of the construct we cloned it before a GFP sequence and expressed it with an IPTG inducible vector. Results see partsregistry page. | The GST-tag was PCR amplified from a pGEX vector with overhang primers including the iGEM restriction sites and then pasted into the iGEM vector. To verify the functionality of the construct we cloned it before a GFP sequence and expressed it with an IPTG inducible vector. Results see partsregistry page. | ||
Revision as of 22:27, 20 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
