Team:Wageningen UR/Safety/One
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====Risk Identification of BioBrick System Inside the Cell Chassis==== | ====Risk Identification of BioBrick System Inside the Cell Chassis==== | ||
- | When infection occurs after all, it is important to note that this part of the project involves the periodic oscillation of an extracellular concentration of a quorum sensing molecule, N-(β-ketocaproyl)-L-Homoserine lactone (an Acyl Homoserine Lactone). The AHL compound is made by the enzyme that | + | When infection occurs after all, it is important to note that this part of the project involves the periodic oscillation of an extracellular concentration of a quorum sensing molecule, N-(β-ketocaproyl)-L-Homoserine lactone (an Acyl Homoserine Lactone). The AHL compound is made by the enzyme that is derived from the ''Vibrio fischeri'' luxI gene. Quorum sensing molecules play a role in many bacterial infections. This means successful transfer of the oscillator BioBrick device to a pathogen could possibly increase its quorum sensing-related pathogenicity. To get an impression where such pathogenic organisms are found we used the environmental BLAST, BLASTN 2.2.25, of NCBI search to find luxI homologous regions in Metagenomes (Zhang, Schwartz, Wagner, & Miller, 2000; Altschul, et al., 1997; [https://2011.igem.org/Team:Wageningen_UR/Safety/Thirteen jump to References]). In marine environments and in farm soils in the U.S.A, environmental representative organisms appeared to have a luxI-like gene. By using other BLASTs (Altschul, et al., 1997; Zhang, Schwartz, Wagner, & Miller, 2000; Altschul, et al., 2005; [https://2011.igem.org/Team:Wageningen_UR/Safety/Thirteen jump to References]) we found human pathogens with a significantly corresponding luxI gene. These pathogens are not representatives of the human gut flora, but they might infect humans by means of contaminated water or food. The pathogens found by the latter BLAST searches include mainly ''Vibrio'' species, for example ''V. cholerae'', capable of causing gastroenteritis. Because of this, the researchers that handle the modified ''E. coli'' will be held on close watch. They should be removed from the lab work in case they have the symptoms of gastroenteritis. Additionally, two fish pathogens and a coral pathogen harbor a gene bearing significant similarity to the luxI gene contained in the Synchroscillator BioBrick system. |
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Revision as of 19:10, 21 September 2011