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| | <h3><i>E.D. FROSTI</i> Project overview</h3> | | <h3><i>E.D. FROSTI</i> Project overview</h3> |
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| - | <table width="590" border="0" cellspacing="0" cellpadding="0"> | + | <center><iframe width="640" height="365" src="http://www.youtube.com/watch?v=3SGGEd-yLpg" frameborder="0" allowfullscreen></iframe></center><br><br> |
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| - | <td align="center" valign="middle"><img src="http://homes.esat.kuleuven.be/~igemwiki/images/project/logo100x100.png" width="100" height="100" style="padding:20px 0 0 0;" /></td>
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| - | <td align="center" valign="bottom"><img src="http://homes.esat.kuleuven.be/~igemwiki/images/project/icon_afp.png" width="23" height="28" /></td>
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| - | <td rowspan="4" align="left" valign="bottom"><img src="http://homes.esat.kuleuven.be/~igemwiki/images/project/arrow_nostim.png" width="80" height="401" style="padding:0 0 30px 0;" /></td>
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| - | <td colspan="3" align="center" valign="middle"><img src="http://homes.esat.kuleuven.be/~igemwiki/images/project/arrows.png" width="257" height="82" style="padding:0 0 20px 0;" /></td>
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| - | <td align="center" valign="middle"><img src="http://homes.esat.kuleuven.be/~igemwiki/images/project/lactose01_cell.png" alt="" width="130" height="130" /></td>
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| - | <td align="center" valign="top"><span class="schematics_txt">Induction of biological properties</span></td>
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| - | <td align="center" valign="middle"><img src="http://homes.esat.kuleuven.be/~igemwiki/images/project/arabinose01_cell.png" width="130" height="130" /></td>
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| - | <td align="center" valign="middle"><img src="http://homes.esat.kuleuven.be/~igemwiki/images/project/lactose_arrow_down.png" width="27" height="62" style="padding:40px 0 30px 0;" /></td>
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| - | <td align="center" valign="middle"><img src="http://homes.esat.kuleuven.be/~igemwiki/images/project/lactose02_death.png" alt="" width="130" height="130" /></td>
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| - | <td align="center" valign="top"><span class="schematics_txt">Application at low temperatures</span></td>
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| - | <td align="center" valign="middle"><img src="http://homes.esat.kuleuven.be/~igemwiki/images/project/arabinose02_death.png" alt="" width="130" height="130" /></td>
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| - | <td colspan="4"><br><p>After stimulation, <i>E.D.Frosti</i> will acquire its desired biological property, i.e. the expression of <b> Ice Nucleation Protein (INP) to induce ice formation</b>, or the expression of a membrane-attached <b> Antifreeze Protein (AFP) to stimulate ice melting</b>. Upon application in the environment at low temperatures, a cell death mechanism will kill off the cells, though the desired biological activity of the proteins on the membranes will remain. This mechanism ensures that the bacteria cannot overgrow the environment. </p>
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| - | <p>Importantly, without any preceding stimulus, no cell death will be induced at low temperatures, so our organism can be safely stored in the fridge. </p><br></td>
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