Week 1

This week we finally started the most important part of our project: the lab work. So of course we had first of all to setup the lab for our needs. Providing schottbottels, flasks, test glasses, ... This week also the first batch of primers arrived so we started the first PCRs.

The PCR for pnikA was done using the Phusion polymerase, the primers pnikA-E,N,X-for and pnikA-S-rev. The annealing temperature was set for 50°C. The template came from the gDNA of the Escherichia coli strain MG1655. (For more information abour the pnikA-system click here).

and prchA

Week 2

Still doing PCRs and start restriction digests.

Week 3

First Biobrick is ready!

Week 4

Week 5

Week 6

Week 7

Week 8

Protocols

Primer