Team:UC Davis

From 2011.igem.org

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<h1>Welcome</h1>
<h1>Welcome</h1>
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<p>Welcome to our page!  Our team is comprised of 6 dedicated individuals: 4 undergraduates and 2 advisors. This will be the third year that iGEM @ UC Davis participates in the competition.  We are hard at work and are looking forward towards the completion of our project.<br><br>This year, we're making libraries of mutant promoters and repressors.  We would like to contribute well characterized mutants to the registry along with characterization of the wildtype parts that we're mutating. We believe that in order for the parts registry to thrive, characterization must be a top priority when adding new parts.  This will ensure that the fundamentals of the registry remain, namely that parts are universal and composable. Along with making mutants, we would like to model their behavior when placed in different simple circuits which could give different behavior (i.e. a switch vs. an oscillator). Once characterized, we will make a predictive algorithm so new mutant interactions can be predicted without the need to physically measure each interaction combination. Read more about it <a href="https://2011.igem.org/Team:UC_Davis/Project">here.</a><br><br>Stay tuned for the upcoming results!</p>
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<p>Welcome to the home of the 2011 iGEM Team at UC Davis!<br><br>
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The four of us have been hard at work on this year's project. Things are coming along well -- we've developed a powerful (and simple) mutagenic PCR protocol, used it on GFP, created and screened a set of LacI promoter mutants, and have selected several TetR and c1 Lambda promoter mutants for characterization.<br><br>
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We are excited for the regional competition in Indianapolis, and are in the process of tidying up the wiki and finalizing our current data for presentation. If you see broken pages (especially ones that aren't fixed within 30 minutes of viewing), please feel free to email all of us at ucdigem2011@gmail.com or <a href="https://2011.igem.org/Team:UC_Davis/Team#Keegan">Keegan Owsley</a> (our designated Code Guy) at keegano@gmail.com.</p>
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<h1 id=About>About Us</h1>
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This is the third time a team at UC Davis has competed in the iGEM Jamboree. This year, we are focusing on foundational advances for BioBrick circuit synthesis. Our project revolves around the streamlining of a mutagenic PCR protocol for the synthesis of mutant libraries from any BioBrick part, from promoters to protein coding genes.<br><br>
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Using this protocol, we set out to create useful mutant libraries of commonly used parts, starting with the repressible LacI, TetR and Lambda c1 promoters. You can read more about our project <a href="https://2011.igem.org/Team:UC_Davis/Project">here.</a><br><br>
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We think that thorough characterization should be a priority for all parts submitted to the <a href="http://partsregistry.org">registry</a>. You can view finished characterization information for our parts on our <a href="https://2011.igem.org/Team:UC_Davis/Project">Project</a> page and review our characterization process on our <a href="https://2011.igem.org/Team:UC_Davis/Data">Data</a> page.
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Revision as of 03:24, 22 September 2011

Our Sponsors

Start a Family

Got a favorite BioBrick? Check our our process for expanding basic parts into part families.

Criteria

View our judging criteria for iGEM 2011 here.

Welcome

Welcome to the home of the 2011 iGEM Team at UC Davis!

The four of us have been hard at work on this year's project. Things are coming along well -- we've developed a powerful (and simple) mutagenic PCR protocol, used it on GFP, created and screened a set of LacI promoter mutants, and have selected several TetR and c1 Lambda promoter mutants for characterization.

We are excited for the regional competition in Indianapolis, and are in the process of tidying up the wiki and finalizing our current data for presentation. If you see broken pages (especially ones that aren't fixed within 30 minutes of viewing), please feel free to email all of us at ucdigem2011@gmail.com or Keegan Owsley (our designated Code Guy) at keegano@gmail.com.

About Us

This is the third time a team at UC Davis has competed in the iGEM Jamboree. This year, we are focusing on foundational advances for BioBrick circuit synthesis. Our project revolves around the streamlining of a mutagenic PCR protocol for the synthesis of mutant libraries from any BioBrick part, from promoters to protein coding genes.

Using this protocol, we set out to create useful mutant libraries of commonly used parts, starting with the repressible LacI, TetR and Lambda c1 promoters. You can read more about our project here.

We think that thorough characterization should be a priority for all parts submitted to the registry. You can view finished characterization information for our parts on our Project page and review our characterization process on our Data page.

Notebook

See what we did on a daily basis in lab. In here you'll find a detailed description of each day's work. Read more.

Take a look at some of the photos we've taken around the lab in our gallery.

Want to know how we did our wet work? You can see our protocols here.



Attributions

We've found that there is an inverse relationship between number of team members and amount of work. For a breakdown of what we worked on, check out our Attributions page.




Safety

For more information on how we worked safely in our lab, please visit our Safety page.

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