Team:DTU-Denmark-2

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<b>Filamentous Fungi</b><br>
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<b>Applications</b><br><br>
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<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly#Plug%20'n'%20Play%20benefits">Here</a> you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the situations where the use of this system is especially advantageous.
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Revision as of 14:30, 19 September 2011



Revolutionizing Synthetic Biology


We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can directly be gathered without the use of restriction enzymes and ligases. This will make synthetic biology faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and fungi ready to plug 'n' play. You can find more information about how this standard works here.

Creating a reporter system

 As proof of concept we in very short time created a reporter system that could be used for anything from monitoring gene expression to determination of protein localization. We chose to express and/or localize fluorescent proteins in the model organism Aspergillus nidulans and the mammalian cell line U-2 OS with great success.
Flexibility

 Standardization entails rigidity. We acknowledge this and have therefore written a guide that allows the user to customize the system. In this way proteins can be assembled seamless, multiple mutations can be introduced in one cloning round, the only thing setting a limit is your creativity.
Applications

 Here you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the situations where the use of this system is especially advantageous.




Achievements
  • We have achieved to create a novel assembly standard system for BioBrick called Plug'n Play with DNA.
  • We submitted 49 new parts and 21 plasmids to the Registry of Standard Biological Parts.
  • We demonstrated the function of our system in the mammalian cell line U2-OS by fluorescence and confocal microscopy.
  • We demonstrated the function of our system in filamentous fungi Aspergilli by fluorescence and microscopy.
  • We demonstrated the function of our system in E. coli by helping the Copenhagen iGEM team.
  • We achieved to characterized two new fungal promoter by use of ß-galactosidase assays and Bradford assays.
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