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Team:UNAM-Genomics Mexico/Parts
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<p>The hydrogen production pathway is composed of four proteins. | <p>The hydrogen production pathway is composed of four proteins. | ||
Our <a class="parts" href="">hydrogenase</a> is the translational fusion of the gene codiffying a | Our <a class="parts" href="">hydrogenase</a> is the translational fusion of the gene codiffying a | ||
| - | <a class="parts" href="">ferredoxin | + | <a class="parts" href="">ferredoxin</a> and <a class="parts" href=""><i>hydA</i></a>. |
<a class="parts" href="">PFOR</a> is a Pyruvate Ferredoxin Oxido-Reductase that helps with the electron flux. | <a class="parts" href="">PFOR</a> is a Pyruvate Ferredoxin Oxido-Reductase that helps with the electron flux. | ||
The other two proteins are <a class="parts" href="">maturases</a>, i.e. enzymes required for the proper assembly of the | The other two proteins are <a class="parts" href="">maturases</a>, i.e. enzymes required for the proper assembly of the | ||
Revision as of 01:35, 14 June 2011
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The hydrogen production pathway is composed of four proteins. Our hydrogenase is the translational fusion of the gene codiffying a ferredoxin and hydA. PFOR is a Pyruvate Ferredoxin Oxido-Reductase that helps with the electron flux. The other two proteins are maturases, i.e. enzymes required for the proper assembly of the hydrogenase. These genes are organized in two operons. The first one comprises the maturase and PFOR and is regulated by the Rizhobium etli promoter pNifH1. The second one is regulated by pLacZ and contains the maturases. Periplasm export tags were added to the hydrogenase and PFOR.
Codon optimization
Our systems final host is R. elti. Given this, we optimized our sequences for R. etli's codon usage.
Periplasm export tags
As a Gramm-negative bacterium, Rhizobium etli has a periplasmatic compartment. Proteins can be exported into it with the appropriate peptide signal.
The Registry
Please visit the registry page if you want to consult our or other team parts speciffic properties.
