Team:DTU-Denmark-2

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<b>Assembly Standard</b><br>
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<b>Creating a reporter system</b><br><br>
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As proof of concept we in very short time created a reporter system that could be used for anything from monitoring gene expression to determination of protein localization. We chose to express and/or localize fluorescent proteins in the model organism <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/fungi"><i>Aspergillus nidulans</i></a> and the <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/mammalian> mammalian cell line U-2 OS</a> with great success.
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Revision as of 21:58, 18 September 2011



Revolutionizing Synthetic Biology


The DTU-Denmark 2 iGEM team has designed a novel standardized assembly system, called “Plug 'n' Play with DNA”, where any biological parts can be gathered without use of restriction enzymes and ligases. This will make synthetic biology faster and assembly of an expression vector possible within a few hours. We have created a library of standardized biological parts for mammalian cells and fungi ready to plug 'n' play.

Creating a reporter system

 As proof of concept we in very short time created a reporter system that could be used for anything from monitoring gene expression to determination of protein localization. We chose to express and/or localize fluorescent proteins in the model organism Aspergillus nidulans and the Mammalian cells
Filamentous Fungi




Achievements
  • We have achieved to create a novel assembly standard system for BioBrick called Plug'n Play with DNA.
  • We submitted 49 new parts and 21 plasmids to the Registry of Standard Biological Parts.
  • We demonstrated the function of our system in the mammalian cell line U2-OS by fluorescence and confocal microscopy.
  • We demonstrated the function of our system in filamentous fungi Aspergilli by fluorescence and microscopy.
  • We demonstrated the function of our system in E. coli by helping the Copenhagen iGEM team.
  • We achieved to characterized two new fungal promoter by use of ß-galactosidase assays and Bradford assays.
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